LOCUS       sequence-115472-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: AP56-5
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(69..91)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(363..438)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(459..808)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     primer_bind     814..836
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     828..845
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     829..845
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    853..873
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
       61 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
      121 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
      181 cttaactatg cggcatcaga gcagattgca ctgagagtgc accatatgcg gtgtgaaata
      241 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
      301 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagaa atttcacaaa
      361 aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc
      421 tatttctagc tctaaaacgt agtcacggcg atgattacaa gacatctcgc aataggaggt
      481 gtttatatag gggcaacaca agaggcggca gacatctcgt gagtttgaca gagtagtata
      541 aagagtgtgt gtgagagggt gtaggcattt cataacatat tttttgtgga ataattttgg
      601 aaaaaaggtg tttttttaat aatagctttt ataagagttt ctcaaaaatt gcatgctttt
      661 tttgaaaagg ttgacaaatt atagtagtac taaagttgct attttcaggt ctcagcagaa
      721 aattcacaac taaaaataaa atttgcaaaa cagagtgtgc gcctttaagg tgtacagttt
      781 tttattcctt tattgctagt tttttttggt tttcccagtc acgacgttgt aaaacgacgg
      841 ccagtgaatt atcaactatg tataataaag ttgtgagact tttttcttgg cggcacaata
      901 aagtttcctc tttttgttgg gtcactcaac gaacacaata caaatatttg agcgctcgca
      961 tttttctcaa cctttcgggt ttaaagcact tcaaaatgga attgaagaga gattgtgaaa
     1021 gtacatc
//
