LOCUS       sequence-115474-        5977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  G protein beta-1 tagged with HaloTag on amino terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5977)
  TITLE     Berlot Lab HaloTag Fusions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HaloTag-G protein beta-1
FEATURES             Location/Qualifiers
     source          1..5977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1075..1084
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3086..3207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3097..3116)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3151..3170
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3393..3848
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3480..3499)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3690..3711
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3915..3934)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4034..4056
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4094..4112)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4180..4284
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4285..4353,4354..5145)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4503..4522)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5316..5904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5805..5824
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagccag ctggcgcgga tatcgccacc
     1081 atgggatccg aaatcggtac aggcttcccc ttcgaccccc attatgtgga agtcctgggc
     1141 gagcgtatgc actacgtcga tgttggaccg cgggatggca cgcctgtgct gttcctgcac
     1201 ggtaacccga cctcgtccta cctgtggcgc aacatcatcc cgcatgtagc accgagtcat
     1261 cggtgcattg ctccagacct gatcgggatg ggaaaatcgg acaaaccaga cctcgattat
     1321 ttcttcgacg accacgtccg ctacctcgat gccttcatcg aagccttggg tttggaagag
     1381 gtcgtcctgg tcatccacga ctggggctca gctctcggat tccactgggc caagcgcaat
     1441 ccggaacggg tcaaaggtat tgcatgtatg gaattcatcc ggcctatccc gacgtgggac
     1501 gaatggccag aattcgcccg tgagaccttc caggccttcc ggaccgccga cgtcggccga
     1561 gagttgatca tcgatcagaa cgctttcatc gagggtgcgc tcccgatggg ggtcgtccgt
     1621 ccgcttacgg aggtcgagat ggaccactat cgcgagccct tcctcaagcc tgttgaccga
     1681 gagccactgt ggcgattccc caacgagctg cccatcgccg gtgagcccgc gaacatcgtc
     1741 gcgctcgtcg aggcatacat gaactggctg caccagtcac ctgtcccgaa gttgttgttc
     1801 tggggcacac ccggcgtact gatccccccg gccgaagccg cgagacttgc cgaaagcctc
     1861 cccaactgca agacagtgga catcggcccg ggattgttct tgctccagga agacaacccg
     1921 gaccttatcg gcagtgagat cgcgcgctgg ctccccgggc tggccggctc cggtgggggc
     1981 gggagcatga gtgagcttga ccagttacgg caggaggccg agcaacttaa gaaccagatt
     2041 cgagacgcca ggaaagcatg tgcagatgca actctctctc agatcacaaa caacatcgac
     2101 ccagtgggaa gaatccaaat gcgcacgagg aggacactgc gggggcacct ggccaagatt
     2161 tacgccatgc actggggcac agactccagg cttctcgtca gtgcctcgca ggatggtaaa
     2221 cttatcatct gggacagcta caccaccaac aaggtccacg ccatccctct gcgctcctcc
     2281 tgggtcatga cctgtgcata tgccccttct gggaactatg tggcctgcgg tggcctggat
     2341 aacatttgct ccatttacaa tctgaaaact cgtgagggga acgtgcgcgt gagtcgtgag
     2401 ctggcaggac acacaggtta cctgtcctgc tgccgattcc tggatgacaa tcagatcgtc
     2461 accagctctg gagacaccac gtgtgccctg tgggacatcg agaccggcca gcagacgacc
     2521 acgtttaccg gacacactgg agatgtcatg agcctttctc ttgctcctga caccagactg
     2581 ttcgtctctg gtgcttgtga tgcttcagcc aaactctggg atgtgcgaga aggcatgtgt
     2641 cggcagacct tcactggcca cgagtctgac atcaatgcca tttgcttctt tccaaatggc
     2701 aatgcatttg ccactggctc agacgacgcc acctgcaggc tgtttgacct tcgtgctgac
     2761 caggagctca tgacttactc ccatgacaac atcatctgcg ggatcacctc tgtctccttc
     2821 tccaagagcg ggcgcctcct ccttgctggg tacgacgact tcaactgcaa cgtctgggat
     2881 gcactcaaag ccgaccgggc aggtgtcttg gctgggcatg acaaccgcgt cagctgcctg
     2941 ggcgtgactg acgatggcat ggctgtggcg acagggtcct gggatagctt cctcaagatt
     3001 tggaactaag gatccgccgg ctaatagtta attaagtagg cggccgcttc cctttagtga
     3061 gggttaatgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     3121 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3181 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3241 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     3301 atccgataag gatcgatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3361 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc
     3421 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     3481 tcctttcgct ttcttccctt cctttctcgc cacgttcgcg ggctttcccc gtcaagctct
     3541 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3601 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3661 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3721 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3781 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     3841 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3901 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     3961 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4021 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     4081 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4141 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4201 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4261 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4321 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4381 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4441 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4501 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4561 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4621 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4681 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4741 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4801 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4861 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4921 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4981 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5041 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5101 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5161 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5221 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5281 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5341 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5401 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5461 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5521 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5581 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5641 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5701 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5761 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5821 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5881 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5941 ttttgctggc cttttgctca catggctcga cagatct
//
