LOCUS       sequence-115476-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: AP56-6
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     92..110
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(148..170)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(442..517)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(538..887)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     primer_bind     893..915
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     907..924
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     908..924
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    932..952
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
       61 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
      121 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
      181 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
      241 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgcac tgagagtgca
      301 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
      361 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      421 acgccagaaa tttcacaaaa agcaccgact cggtgccact ttttcaagtt gataacggac
      481 tagccttatt ttaacttgct atttctagct ctaaaacgta gtcacggcga tgattacaag
      541 acatctcgca ataggaggtg tttatatagg ggcaacacaa gaggcggcag acatctcgtg
      601 agtttgacag agtagtataa agagtgtgtg tgagagggtg taggcatttc ataacatatt
      661 ttttgtggaa taattttgga aaaaaggtgt ttttttaata atagctttta taagagtttc
      721 tcaaaaattg catgcttttt ttgaaaaggt tgacaaatta tagtagtact aaagttgcta
      781 ttttcaggtc tcagcagaaa attcacaact aaaaataaaa tttgcaaaac agagtgtgcg
      841 cctttaaggt gtacagtttt ttattccttt attgctagtt ttttttggtt ttcccagtca
      901 cgacgttgta aaacgacggc cagtgaatta tcaactatgt ataataaagt tgtgagactt
      961 ttttcttggc ggcacaataa agtttcctct ttttgttggg tcactcaacg aacacaatac
     1021 aaatattt
//
