LOCUS       sequence-115482-         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 4-6 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX2-45
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(385..402)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(385..401)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(394..416)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      465..551
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcacccggca accttgggta gcagcgaagt cgaggcattt ctgtcctggc tggtctagac
       61 gtctcgccat ggtcctggca cagcaccggc aacagccgct gcaccgtttc gagcgcttgc
      121 ttgccgccaa tgttgctggc gatagccacc acttggtccg gggtcaggcc atggtcctgg
      181 cacagcaccg gcaacagccg ctgcaccgtt tcgagcgctt gcttgccgcc accgttgctg
      241 gcgatagcca ccacttggtc cggggtcagg ccatggtcct ggcacagcac cggcaacagc
      301 cgctgcaccg tttcgagcgc ttgcttgccg ccattgttgc tggcgatagc caccacttgg
      361 tccggggtca gggagacgct taagactggc cgtcgtttta caacgtcgtg actgggaaaa
      421 catccatgct agcgttaacg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
      481 ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
      541 gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc ggagggtggc
      601 gggcaggacg cccgccataa actgccaggc atcaaactaa gcagaaggcc atcctga
//
