LOCUS       sequence-115487-        5170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  G protein gamma-2 tagged with HaloTag on amino terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5170)
  TITLE     Berlot Lab HaloTag Fusions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HaloTag-G protein gamma-2
FEATURES             Location/Qualifiers
     source          1..5170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1075..1084
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2279..2400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2290..2309)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2344..2363
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2586..3041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2673..2692)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2883..2904
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3108..3127)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3227..3249
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3287..3305)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3373..3477
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3478..3546,3547..4338)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3696..3715)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4509..5097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4998..5017
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagccag ctggcgcgga tatcgccacc
     1081 atgggatccg aaatcggtac aggcttcccc ttcgaccccc attatgtgga agtcctgggc
     1141 gagcgtatgc actacgtcga tgttggaccg cgggatggca cgcctgtgct gttcctgcac
     1201 ggtaacccga cctcgtccta cctgtggcgc aacatcatcc cgcatgtagc accgagtcat
     1261 cggtgcattg ctccagacct gatcgggatg ggaaaatcgg acaaaccaga cctcgattat
     1321 ttcttcgacg accacgtccg ctacctcgat gccttcatcg aagccttggg tttggaagag
     1381 gtcgtcctgg tcatccacga ctggggctca gctctcggat tccactgggc caagcgcaat
     1441 ccggaacggg tcaaaggtat tgcatgtatg gaattcatcc ggcctatccc gacgtgggac
     1501 gaatggccag aattcgcccg tgagaccttc caggccttcc ggaccgccga cgtcggccga
     1561 gagttgatca tcgatcagaa cgctttcatc gagggtgcgc tcccgatggg ggtcgtccgt
     1621 ccgcttacgg aggtcgagat ggaccactat cgcgagccct tcctcaagcc tgttgaccga
     1681 gagccactgt ggcgattccc caacgagctg cccatcgccg gtgagcccgc gaacatcgtc
     1741 gcgctcgtcg aggcatacat gaactggctg caccagtcac ctgtcccgaa gttgttgttc
     1801 tggggcacac ccggcgtact gatccccccg gccgaagccg cgagacttgc cgaaagcctc
     1861 cccaactgca agacagtgga catcggcccg ggattgttct tgctccagga agacaacccg
     1921 gaccttatcg gcagtgagat cgcgcgctgg ctccccgggc tggccggctc cggtgggggc
     1981 gggagcatgg ccagcaacaa caccgccagc atagcacaag ccaggaaact ggtagaacag
     2041 ctgaagatgg aagccaacat cgataggata aaggtgtcca aggcagctgc agatttgatg
     2101 gcctactgtg aagcgcatgc caaggaagat cccctcctga cacctgttcc ggcttcagaa
     2161 aacccattta gggagaagaa gtttttctgt gctatccttt aaggatccgc cggctaatag
     2221 ttaattaagt aggcggccgc ttccctttag tgagggttaa tgcttcgagc agacatgata
     2281 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2341 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2401 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
     2461 taaagcaagt aaaacctcta caaatgtggt aaaatccgat aaggatcgat ccgggctggc
     2521 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     2581 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     2641 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     2701 cgccacgttc gcgggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     2761 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     2821 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     2881 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     2941 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3001 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
     3061 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     3121 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3181 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3241 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     3301 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     3361 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3421 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3481 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3541 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3601 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3661 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3721 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3781 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3841 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3901 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3961 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4021 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4081 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4141 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4201 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4261 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4321 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4381 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4441 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4501 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4561 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4621 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     4681 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4741 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4801 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4861 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4921 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4981 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5041 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5101 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggct
     5161 cgacagatct
//
