LOCUS       sequence-115498-        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PTS2-ONL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pONL-N1_PTS2
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      164..173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             170..823
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             170..823
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tcgccaccat gcagagattg caagtggtcc tagggcacct
      121 ccgaggaccg tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcgt
      181 gatcaagccc gagatgaaga tgaggtacta catggacggc tccgtcaatg ggcatgagtt
      241 cacaatcgag ggtgagggca caggcagacc ttacgaggga catcaggaga tgacactgcg
      301 cgtcacaatg gccgagggcg ggccaatgcc tttcgccttc gacctggtgt cccacgtgtt
      361 ctgttacggc cacagagttt ttactaaata tccagaagag atcccagact atttcaagca
      421 ggcctttcct gagggcctgt cctgggagag gtccctggag ttcgaggacg gcggctccgc
      481 ctccgtgagc gcccacatca gcctgagggg caacaccttc taccacaagt ccaagttcac
      541 cggcgtgaac ttccccgccg acggccccat catgcagaac cagagcgtgg actgggagcc
      601 ctccaccgag aagatcaccg ccagcgacgg cgtgctgaag ggcgacgtga ccatgtacct
      661 gaagctggag ggcggcggca accacaagtg ccagatgaag accacctaca aggccgccaa
      721 ggagatcctg gagatgcccg gcgaccacta catcggccac aggctggtga ggaagaccga
      781 gggcaacatc accgagcagg tggaggacgc cgtggcccac tccggtacca aggtgtacga
      841 ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg tgggctcgct gcaagcaaat
      901 gaacgtgctg gactccttca tcaactacta tgattccgag aagcacgccg agaacgccgt
      961 gatttttctg catggtaacg ctacctccag ctacctgtgg aggcacgtcg tgcctcacat
     1021 cgagcccgtg gctagatgca tcatccctga tctgatcgga atgggtaagt ccggcaagag
     1081 c
//
