LOCUS       sequence-115505-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mito-ONL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pONL-N1_Mito
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..119
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      147..156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             153..806
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             153..806
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcatgtcc gtcctgacgc
       61 cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg cgcgccaaga
      121 tccattcgtt gggggatcca ccggtcgcca ccatggtgag cgtgatcaag cccgagatga
      181 agatgaggta ctacatggac ggctccgtca atgggcatga gttcacaatc gagggtgagg
      241 gcacaggcag accttacgag ggacatcagg agatgacact gcgcgtcaca atggccgagg
      301 gcgggccaat gcctttcgcc ttcgacctgg tgtcccacgt gttctgttac ggccacagag
      361 tttttactaa atatccagaa gagatcccag actatttcaa gcaggccttt cctgagggcc
      421 tgtcctggga gaggtccctg gagttcgagg acggcggctc cgcctccgtg agcgcccaca
      481 tcagcctgag gggcaacacc ttctaccaca agtccaagtt caccggcgtg aacttccccg
      541 ccgacggccc catcatgcag aaccagagcg tggactggga gccctccacc gagaagatca
      601 ccgccagcga cggcgtgctg aagggcgacg tgaccatgta cctgaagctg gagggcggcg
      661 gcaaccacaa gtgccagatg aagaccacct acaaggccgc caaggagatc ctggagatgc
      721 ccggcgacca ctacatcggc cacaggctgg tgaggaagac cgagggcaac atcaccgagc
      781 aggtggagga cgccgtggcc cactccggta ccaaggtgta cgaccccgag caacgcaaac
      841 gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct
      901 tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta
      961 acgctacctc cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat
     1021 gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcata
//
