LOCUS       sequence-115513-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NLS-CNL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pCNL-N1_3xNLS
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..78
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             82..102
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             106..126
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(157..159,160..162,163..675)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(202..223)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(463..482)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     810..831
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagccaccat gctggtacca
       61 aaaaagaaga gaaaggttgt accaaaaaag aagagaaagg ttgtaccaaa aaagaagaga
      121 aaggttttcg aagggccgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
      181 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      241 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      301 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gtcctggggc
      361 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      421 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      481 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      541 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctactttagc
      601 gacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
      661 cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
      721 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
      781 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      841 gggggtacca aggtgtacga ccccgagcag aggaagagga tgatcaccgg cccccagtgg
      901 tgggccaggt gcaagcagat gaacgtgctg gacagcttca tcaactacta cgacagcgag
      961 aagcacgccg agaacgccgt gatcttcctg cacggcaacg ccactagcag ctacctgtgg
     1021 aggcacgtgg tgccccacat cgagcccgtg gccaggtgca tcatccccga tctgatcggc
     1081 atgggcaaga gcggcaagag
//
