LOCUS       sequence-11552-e        9922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 9922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pT7-EGFP-C1-HsTNRC6Biso1
FEATURES             Location/Qualifiers
     source          1..9922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6710..6831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6747..6766)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6801..6820
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6838..7293)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6975..6996)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7187..7206
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7320..7424
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7426..7783
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7426..7617
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7449..7469)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7634..7769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7818..8612
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7818..8612
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7872..7891)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8482..8501
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8800..8819)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8844..8891
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9220..9808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9709..9728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctcaagcttc gatgagagag aaggagcaag aaagggaaga
     1441 acagttaatg gaagacaaga aaaggaagaa agaggataaa aagaaaaaag aagccactca
     1501 gaaggtcacg gaacaaaaaa ccaaagtgcc cgaagtgacg aaaccaagtt taagccaacc
     1561 aacggccgcc agcccaattg gcagctctcc atcgccacca gtcaatggtg gcaacaatgc
     1621 caaaagggtg gcagtgccga acggacaacc gccaagcgcc gcccgctaca tgcctcggga
     1681 ggtgccgccg cgattccgtt gccagcagga ccacaaagtg ttactaaaac gtgggcagcc
     1741 ccctccaccg tcctgcatgc tccttggggg tggggcaggg cctcctccct gcacagcacc
     1801 tggagcaaac ccaaacaacg cacaagtgac aggagcgctg ctgcagagtg agagtgggac
     1861 tgcgccagac tcaacccttg gaggtgctgc tgcttcaaat tatgcaaatt ccacttgggg
     1921 ctcgggagcc tcctccaaca acggcacctc ccccaaccca attcacatct gggacaaggt
     1981 gattgtagac gggtctgaca tggaagagtg gccttgtatt gccagcaaag acactgaatc
     2041 ttcttccgaa aacaccaccg ataacaacag tgcctcgaac cctggctctg agaagagcac
     2101 tctgccagga agcaccacta gtaacaaagg aaaagggagc cagtgccagt ctgcaagttc
     2161 tgggaacgaa tgtaatcttg gggtctggaa atctgaccct aaggctaaat ctgttcaatc
     2221 ttccaactct actacagaga acaacaatgg actaggaaat tggaggaatg tgagtggtca
     2281 ggatagaatt ggacctggct ctggcttcag caactttaac ccaaatagca acccatctgc
     2341 ctggccagca ctggtccaag aaggaacttc taggaaaggg gcattggaaa cagataatag
     2401 taattccagt gcacaggtta gcacagtagg tcagacatcc agggaacagc agtcaaagat
     2461 ggaaaatgcg ggtgttaatt ttgttgtctc tggcagagaa caggctcaaa ttcataacac
     2521 tgatggacca aaaaatggaa acactaactc cttgaactta agttcaccaa accccatgga
     2581 gaataaggga atgccctttg gaatgggctt ggggaacacc tccaggagca ctgatgcccc
     2641 ttcacaaagc actggagatc gaaagactgg gagtgttgga tcttggggtg cagctagggg
     2701 gccttctgga actgacacag tctctggaca aggcaattct ggaaacaatg ggaacaatgg
     2761 aaaagagaga gaggactcct ggaaaggagc ttctgttcag aaatcaactg ggtcaaaaaa
     2821 tgactcttgg gacaacaata acaggtctac gggtgggtcc tggaactttg gcccccagga
     2881 ctctaatgac aacaaatggg gtgaagggaa caaaatgaca tctggggtct ctcagggaga
     2941 atggaaacag ccgactgggt ctgatgagtt gaaaattgga gaatggagtg gtccaaacca
     3001 accaaattct agcactggag catgggacaa tcaaaagggc caccccctcc ctgaaaacca
     3061 aggcaatgcc caggctccct gttggggaag atcttccagc tccacaggaa gtgaagttgg
     3121 aggtcaaagc actggaagca accacaaagc aggaagtagt gacagtcata actctggccg
     3181 tcggtcgtac aggcccacac atcctgattg tcaggctgtc ttgcagactc ttttgagccg
     3241 aactgatttg gaccccaggg tgctctcaaa cactggctgg ggccaaactc aaattaagca
     3301 ggacacagtg tgggacattg aagaggtgcc aaggcctgag gggaaatctg acaaaggaac
     3361 tgaggggtgg gagagcgctg ccacacagac caagaactca gggggctggg gagatgcacc
     3421 cagccaaagc aatcaaatga agtctggatg gggggagctc tcagcctcta cagagtggaa
     3481 agaccccaag aacacaggag gctggaatga ctacaagaac aacaactctt ccaactgggg
     3541 aggaggacga cctgatgaaa agacaccttc ctcttggaat gagaatccca gcaaggatca
     3601 ggggtgggga ggtggacgcc agcccaatca aggatggtct tctggaaaga atggttgggg
     3661 ggaggaagtc gatcagacaa aaaacagcaa ttgggaaagt tctgcaagta aacctgtgtc
     3721 tgggtggggt gaaggagggc agaatgaaat cgggacttgg ggtaatggtg gcaatgcaag
     3781 cctagcttca aaaggtgggt gggaggattg caaaagatcc ccagcatgga atgagacggg
     3841 ccgacagccc aattcctgga ataaacaaca ccaacagcag cagcccccac agcagccgcc
     3901 gccaccacaa ccagaggctt ctggttcgtg gggaggccca cccccaccac ctccaggcaa
     3961 cgttcgacct tccaattcca gctggagcag cgggccacag cctgcaacac ctaaggatga
     4021 ggaacccagt ggttgggaag agccatcccc acagtcaatt agtcggaaaa tggacattga
     4081 tgatggcact tcagcatggg gagaccctaa cagttataac tacaagaatg tgaatctgtg
     4141 ggataagaat tcccaagggg gcccagcacc tcgagaacca aacctgccca ccccaatgac
     4201 cagtaaatcg gcatcagatt ccaaatctat gcaagacggc tggggggaga gtgacgggcc
     4261 agtcacagga gctcgccatc ccagctggga agaggaggag gatggaggag tctggaacac
     4321 cactggctct cagggcagtg cttcctccca caactcagca agctggggac aaggaggaaa
     4381 gaaacaaatg aagtgctcac tcaaaggagg aaacaatgat tcatggatga atcctcttgc
     4441 caaacagttt tcaaatatgg gattgctgag tcagactgaa gataatccaa gcagcaaaat
     4501 ggatttgtct gtaggaagcc tttcagataa aaaatttgat gtggacaagc gagcgatgaa
     4561 tctcggggat tttaatgata tcatgaggaa ggatcgatct gggttccgtc cacctaattc
     4621 caaagacatg ggaaccacag atagtgggcc ttattttgag aagggcggta gtcgtggttt
     4681 gtttggaaac agcacagcac aatcgagagg tctgcacaca cccgtgcggc cactaaattc
     4741 ttctcccagt ctccgggcgc aagtgcctcc ccagtttatt tccccccagg tttctgcctc
     4801 aatgctcaag cagtttccca acagtggcct gagtccaggt cttttcaatg tggggcccca
     4861 gttatctcct caacaaattg ccatgctgag ccagcttcca caaattcccc agtttcagtt
     4921 ggcatgtcag cttctcttgc agcagcagca acagcagcgg ttgttacaga accagagaaa
     4981 gatttctcaa gctgtacgcc aacagcaaga gcagcagctg gctcgaatgg tgagtgcact
     5041 gcagcagcag cagcagcagc agaggcagcc aggcatgaag cactcgccct ctcatcctgt
     5101 tgggcccaag ccgcatctgg acaacatggt acccaacgca ttgaatgtgg ggctcccaga
     5161 ccttcaaacc aaagggccaa tacctggata tggttctggc ttcagctctg gcggcatgga
     5221 ctatggcatg gttggtggga aggaggctgg aaccgagtct cgctttaaac agtggacctc
     5281 catgatggag gggctgccct ctgtagccac acaggaagcc aatatgcaca aaaatggcgc
     5341 tatagtggcc cctggtaaaa cccggggagg gtcaccgtac aaccagtttg atatcatccc
     5401 tggtgacaca ctgggtggcc atacgggtcc tgctggtgat agctggttac ctgccaaatc
     5461 tccaccaaca aataaaatcg gaagtaaatc cagcaatgcc agttggcctc cagaattcca
     5521 accaggagtg ccatggaaag gtatccaaaa cattgaccct gaatctgacc cctatgtcac
     5581 cccaggaagt gtgctggggg gtacagccac atctcccatt gtagatactg accaccaact
     5641 gctgcgggat aacaccacag ggtctaattc ttccctcaac acctcgctgc cttcacctgg
     5701 tgcctggccc tacagtgcct ctgacaactc ctttaccaac gttcatagca cttcagcaaa
     5761 gttccctgat tacaaatcaa catggtcccc agatcccata ggacacaacc ccactcatct
     5821 ctccaacaag atgtggaaaa accatatttc ctccaggaac actgcaccgc tgccccgccc
     5881 acctcctggt ctgaccaacc ccaaaccatc atctccctgg agcagcacag caccccgatc
     5941 agtcaggggg tgggggacac aggactcacg gctcgcctcg gcctctacct ggagtgatgg
     6001 tggctcagtt cgtcctagtt actggctggt tcttcacaat ctcaccccac agattgatgg
     6061 gtcaaccttg agaacgatct gcatgcagca tggcccactg ctgacattcc atctgaatct
     6121 aacccagggc actgccctga tccgatacag caccaaacag gaggcggcca aggcccaaac
     6181 tgcactgcac atgtgtgtgt tgggaaacac taccatcctt gctgagtttg ccactgatga
     6241 tgaagtcagc cgctttctgg cacaagctca gccccctaca cctgcagcaa ccccaagtgc
     6301 gccagctgcg gggtggcagt cgctggagac cggccagaac cagtcagatc ccgtgggacc
     6361 tgctctgaat ctttttggtg ggtccactgg gctcgggcag tggagcagca gcgctggtgg
     6421 cagcagcggg gccgatcttg ctggcgcttc attgtggggg cccccaaact attcttctag
     6481 cttatgggga gtcccaacgg tggaagatcc ccataggatg ggcagccctg ctcctttact
     6541 acctggtgac cttctgggag gagggtcgga ttcaatctga ggatccaccg gatctagata
     6601 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     6661 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     6721 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6781 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa
     6841 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     6901 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     6961 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     7021 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     7081 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     7141 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     7201 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     7261 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     7321 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7381 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     7441 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7501 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7561 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7621 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7681 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7741 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     7801 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     7861 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     7921 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     7981 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     8041 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     8101 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     8161 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     8221 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     8281 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     8341 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     8401 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     8461 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     8521 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     8581 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     8641 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     8701 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8761 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     8821 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     8881 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     8941 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     9001 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     9061 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9121 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9181 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9241 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9301 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9361 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9421 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9481 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9541 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9601 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9661 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9721 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9781 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9841 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9901 taaccgtatt accgccatgc at
//
