LOCUS       sequence-115528-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary T-DNA vector expressing EGFP and neomycin phosphotransferase 
            II (NPTII); for Agrobacterium tumefaciens-mediated transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Maximova S, Miller C, Antunez de Mayolo G, Pishak S, Young A, 
            Guiltinan MJ
  TITLE     Stable transformation of Theobroma cacao L. and influence of matrix 
            attachment regions on GFP expression.
  JOURNAL   Plant Cell Rep. 2003 Jun;21(9):872-83. Epub 2003 Apr 3.
  PUBMED    12789505
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2003-06"; volume: "21(9)"; pages: "872-83. Epub 2003 
            Apr 3"
COMMENT     Sequence Label: pGH00.0126
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..42
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        166..345
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        198..381
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(456..475)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
       61 tgaaggcggg aaacgacaat ctgatcatga gcggagaatt aagggagtca cgttatgacc
      121 cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc gcaacgttga
      181 aggagccact cagccgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc
      241 attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa
      301 aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac
      361 tctcaatcca aataatctgc accggatctg gatcgtttcg catgattgaa caagatggat
      421 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
      481 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
      541 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
      601 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
      661 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
      721 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
      781 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
      841 ggatggaagc cggtcttgtc gatcaggatg atctgg
//
