LOCUS       sequence-115529-       15607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary T-DNA vector expressing EGFP and neomycin phosphotransferase 
            II (NPTII); for Agrobacterium tumefaciens-mediated transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15607)
  AUTHORS   Maximova S, Miller C, Antunez de Mayolo G, Pishak S, Young A, 
            Guiltinan MJ
  TITLE     Stable transformation of Theobroma cacao L. and influence of matrix 
            attachment regions on GFP expression.
  JOURNAL   Plant Cell Rep. 2003 Jun;21(9):872-83. Epub 2003 Apr 3.
  PUBMED    12789505
REFERENCE   2  (bases 1 to 15607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2003-06"; volume: "21(9)"; pages: "872-83. Epub 2003 
            Apr 3"
COMMENT     Sequence Label: pGH00.0126
FEATURES             Location/Qualifiers
     source          1..15607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..726
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(914..931)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1165..1184)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            1407..1516
                     /note="incP origin of transfer"
     rep_origin      2046..2757
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  3380..4147
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             4339..5133
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             5432..6580
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    8176..8200
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(8447..8468)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8659..8678
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8803..8825
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8817..8834
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8818..8834
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    8865..9039
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(join(9077..9790,9791..9793,9794..9796))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9080..9790,9791..9793,9794..9796))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9122..9143)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9471..9490
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9730..9751
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      9793..9802
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9902..9947)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     polyA_signal    10664..10838
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11042..11836)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(11936..11981)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(12732..12748)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12732..12748)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12745..12767)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12756..12772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12780..12786,12787..12804,12805..12810))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12825..12846
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(13395..13647)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(14037..14831)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(14852..15035)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(14888..15067)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    15191..15215
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cgattgaacg cgcggattct ttatcactga taagttggtg gacatattat gtttatcagt
       61 gataaagtgt caagcatgac aaagttgcag ccgaatacag tgatccgtgc cgccctggac
      121 ctgttgaacg aggtcggcgt agacggtctg acgacacgca aactggcgga acggttgggg
      181 gttcagcagc cggcgcttta ctggcacttc aggaacaagc gggcgctgct cgacgcactg
      241 gccgaagcca tgctggcgga gaatcatacg cattcggtgc cgagagccga cgacgactgg
      301 cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg cgctgctcgc ctaccgcgat
      361 ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc agatggaaac ggccgacgcg
      421 cagcttcgct tcctctgcga ggcgggtttt tcggccgggg acgccgtcaa tgcgctgatg
      481 acaatcagct acttcactgt tggggccgtg cttgaggagc aggccggcga cagcgatgcc
      541 ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc cgctgttgcg ggccgcgata
      601 gacgccttcg acgaagccgg tccggacgca gcgttcgagc agggactcgc ggtgattgtc
      661 gatggattgg cgaaaaggag gctcgttgtc aggaacgttg aaggaccgag aaagggtgac
      721 gattgatcag gaccgctgcc ggagcgcaac ccactcacta cagcagagcc atgtagacaa
      781 catcccctcc ccctttccac cgcgtcagac gcccgtagca gcccgctacg ggctttttca
      841 tgccctgtcc tagcgtccaa gcctcacggc cgcgctcggc ctctctggcg gccttctggc
      901 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      961 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1021 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1081 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1141 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1201 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1261 gaagcgtggc gcttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg
     1321 gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt
     1381 ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc
     1441 gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct
     1501 ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggat ggctgatgaa
     1561 accaagccaa ccaggaaggg cagcccacct atcaaggtgt actgccttcc agacgaacga
     1621 agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc
     1681 gtcggccagg gctacaaaat cacgggcgtc gtggactatg agcacgtccg cgagctggcc
     1741 cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga aactctggcc accgacgacc
     1801 cgcgcacggc gcggttcggt gatgccacaa tcctcgcgct gctggcgaag atcgaagaga
     1861 agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac
     1921 ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc
     1981 tccatcaaga agagcgactt cgcggagctg gtgaagtaca tcaccgacga gcaaggcaag
     2041 accgagcgcc tttccgacgc tcaccgggct ggttgccctc gccgctgggc tggcggccgt
     2101 ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg
     2161 ccggcgttgt ggatacctcg cggaaaactt ggccctcact gacagatgag gggcggacgt
     2221 tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc aggctcgatt
     2281 tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct gattttacgc
     2341 gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt attgacactt
     2401 gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg ggcagagtgc
     2461 tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggca gaaaatccag
     2521 catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt
     2581 taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg
     2641 cacgccgaag gggggtgccc ccccttctcg aaccctcccg gcccgctaac gcgggcctcc
     2701 catcccccca ggggctgcgc ccctcggccg cgaacggcct caccccaaaa atggcagcgc
     2761 tggcagtcct tgccattgcc gggatcgggg cagtaacggg atgggcgatc agcccgagcg
     2821 cgacgcccgg aagcattgac gtgccgcagg tgctggcatc gacattcagc gaccaggtgc
     2881 cgggcagtga gggcggcggc ctgggtggcg gcctgccctt cacttcggcc gtcggggcat
     2941 tcacggactt catggcgggg ccggcaattt ttaccttggg cattcttggc atagtggtcg
     3001 cgggtgccgt gctcgtgttc gggggtgcga taaacccagc gaaccatttg aggtgatagg
     3061 taagattata ccgaggtatg aaaacgagaa ttggaccttt acagaattac tctatgaagc
     3121 gccatattta aaaagctacc aagacgaaga ggatgaagag gatgaggagg cagattgcct
     3181 tgaatatatt gacaatactg ataagataat atatctttta tatagaagat atcgccgtat
     3241 gtaaggattt cagggggcaa ggcataggca gcgcgcttat caatatatct atagaatggg
     3301 caaagcataa aaacttgcat ggactaatgc ttgaaaccca ggacaataac cttatagctt
     3361 gtaaattcta tcataattgg gtaatgactc caacttattg atagtgtttt atgttcagat
     3421 aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc gacttccgtc
     3481 ccagccgtgc catgtgctgc ctcagattca ggttatgccg ctcaattcgc tgcgtatatc
     3541 gcttgctgat tacgtgcagc tttcccttca ggcgggattc atacagcggc cagccatccg
     3601 tcatccatat caccacgtca aagggtgaca gcaggctcat aagacgcccc agcgtcgcca
     3661 tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg gagactgtca tacgcgtaaa
     3721 acagccagcg ctggcgcgat ttagccccga catagcccca ctgttcgtcc atttccgcgc
     3781 agacgatgac gtcactgccc ggctgtatgc gcgaggttac cgactgcggc ctgagttttt
     3841 taagtgacgt aaaatcgtgt tgaggccaac gcccataatg cgggctgttg cccggcatcc
     3901 aacgccattc atggccatat caatgatttt ctggtgcgta ccgggttgag aagcggtgta
     3961 agtgaactgc agttgccatg ttttacggca gtgagagcag agatagcgct gatgtccggc
     4021 ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga
     4081 aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag
     4141 catcacccat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     4201 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
     4261 tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa
     4321 agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
     4381 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
     4441 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
     4501 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
     4561 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
     4621 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
     4681 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
     4741 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
     4801 gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt
     4861 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
     4921 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
     4981 gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa
     5041 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
     5101 aaatattata ttttactgga tgaattgttt tagtacctag atgtggcgca acgatgccgg
     5161 cgacaagcag gagcgcaccg acttcttccg catcaagtgt tttggctctc aggccgaggc
     5221 ccacggcaag tatttgggca aggggtcgct ggtattcgtg cagggcaaga ttcggaatac
     5281 caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg ataaggtgga
     5341 ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca ttgccccggc
     5401 gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc ggaaggcata
     5461 caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca tcgcaagccg
     5521 caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc
     5581 tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc ccgcgccatc
     5641 ggccgccgtg gagcgttcgc gtcgtctcga acaggaggcg gcaggtttgg cgaagtcgat
     5701 gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg gcgaggacct
     5761 ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga agcagcagat
     5821 caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat
     5881 gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga
     5941 ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca cctacaccgg
     6001 cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg agtacgcgaa
     6061 gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc aggacctggg
     6121 ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc
     6181 gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca
     6241 ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga
     6301 ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg agaagtaccg
     6361 caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc gggagccgta
     6421 cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa
     6481 gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc tggtggaaca
     6541 cgcctgggtc aatgatgacc tggtgcattg caaacgctag ggccttgtgg ggtcagttcc
     6601 ggctgggggt tcagcagcca gcgctttact ggcatttcag gaacaagcgg gcactgctcg
     6661 acgcacttgc ttcgctcagt atcgctcggg acgcacggcg cgctctacga actgccgata
     6721 aacagaggat taaaattgac aattgtgatt aaggctcaga ttcgacggct tggagcggcc
     6781 gacgtgcagg atttccgcga gatccgattg tcggccctga agaaagctcc agagatgttc
     6841 gggtccgttt acgagcacga ggagaaaaag cccatggagg cgttcgctga acggttgcga
     6901 gatgccgtgg cattcggcgc ctacatcgac ggcgagatca ttgggctgtc ggtcttcaaa
     6961 caggaggacg gccccaagga cgctcacaag gcgcatctgt ccggcgtttt cgtggagccc
     7021 gaacagcgag gccgaggggt cgccggtatg ctgctgcggg cgttgccggc gggtttattg
     7081 ctcgtgatga tcgtccgaca gattccaacg ggaatctggt ggatgcgcat cttcatcctc
     7141 ggcgcactta atatttcgct attctggagc ttgttgttta tttcggtcta ccgcctgccg
     7201 ggcggggtcg cggcgacggt aggcgctgtg cagccgctga tggtcgtgtt catctctgcc
     7261 gctctgctag gtagcccgat acgattgatg gcggtcctgg gggctatttg cggaactgcg
     7321 ggcgtggcgc tgttggtgtt gacaccaaac gcagcgctag atcctgtcgg cgtcgcagcg
     7381 ggcctggcgg gggcggtttc catggcgttc ggaaccgtgc tgacccgcaa gtggcaacct
     7441 cccgtgcctc tgctcacctt taccgcctgg caactggcgg ccggaggact tctgctcgtt
     7501 ccagtagctt tagtgtttga tccgccaatc ccgatgccta caggaaccaa tgttctcggc
     7561 ctggcgtggc tcggcctgat cggagcgggt ttaacctact tcctttggtt ccgggggatc
     7621 tcgcgactcg aacctacagt tgtttcctta ctgggctttc tcagccccag atctggggtc
     7681 gatcagccgg ggatgcatca ggccgacagt cggaacttcg ggtccccgac ctgtaccatt
     7741 cggtgagcaa tggatagggg agttgatatc gtcaacgttc acttctaaag aaatagcgcc
     7801 actcagcttc ctcagcggct ttatccagcg atttcctatt atgtcggcat agttctcaag
     7861 atcgacagcc tgtcacggtt aagcgagaaa tgaataagaa ggctgataat tcggatctct
     7921 gcgagggaga tgatatttga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     7981 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     8041 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
     8101 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
     8161 ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact
     8221 taataacaca ttgcggacgt ttttaatgta ctggggtggt ttttcttttc accagtgaga
     8281 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     8341 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttccgaaa tcggcaaaat
     8401 cccttataaa tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa
     8461 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     8521 cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa
     8581 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     8641 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgccattc aggctgcgca
     8701 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     8761 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     8821 aaacgacggc cagtgaattc gagctcggta cccggaattc gggggatctg gattttagta
     8881 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     8941 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
     9001 cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgc atgccaattc
     9061 tagagtcgcg gccgctttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     9121 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
     9181 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     9241 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     9301 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
     9361 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     9421 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     9481 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     9541 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
     9601 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
     9661 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
     9721 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
     9781 cgcccttgct caccatggtg gcgaccggta cccggggatc ctctagatgt agttgtagaa
     9841 tgtaaaatgt aatgttgttg ttgtttgttg ttgttgttgg taattgttgt aaaaatacgt
     9901 acctctccga atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg
     9961 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
    10021 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
    10081 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
    10141 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
    10201 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
    10261 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttca
    10321 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
    10381 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
    10441 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
    10501 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
    10561 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
    10621 agagtgtcgt gctccaccat gttggcaagc ttggaattcg ggggatctgg attttagtac
    10681 tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata
    10741 catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc
    10801 ccttatctgg gaactactca cacattatta tggagaaact cgagcttgca tgccaattct
    10861 agaattccgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag
    10921 ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg
    10981 aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg
    11041 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
    11101 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
    11161 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
    11221 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
    11281 cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
    11341 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
    11401 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
    11461 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
    11521 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
    11581 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
    11641 gcgctgcctc gtcctggagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
    11701 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
    11761 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
    11821 catcttgttc aatcatgcga aacgatccgg ggaattctag atgtagttgt agaatgtaaa
    11881 atgtaatgtt gttgttgttt gttgttgttg ttggtaattg ttgtaaaaat acgtacctct
    11941 ccgaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg
    12001 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg
    12061 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
    12121 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
    12181 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
    12241 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
    12301 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttcacatcaa
    12361 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
    12421 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
    12481 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
    12541 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
    12601 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
    12661 tcgtgctcca ccatgttggc aagcttatcg ataccgtcga cctgcaggca tgcaagcttg
    12721 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    12781 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    12841 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    12901 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcc aaagacaaaa
    12961 gggcgacatt caaccgattg agggagggaa ggtaaatatt gacggaaatt attcattaaa
    13021 ggtgaattat caccgtcacc gacttgagcc atttgggaat tagagccagc aaaatcacca
    13081 gtagcaccat taccattagc aaggccggaa acgtcaccaa tgaaaccatc gatagcagca
    13141 ccgtaatcag tagcgacaga atcaagtttg cctttagcgt cagactgtag cgcgttttca
    13201 tcggcatttt cggtcatagc ccccttatta gcgtttgcca tcttttcata atcaaaatca
    13261 ccggaaccag agccaccacc ggaaccgcct ccctcagagc cgccaccctc agaaccgcca
    13321 ccctcagagc caccaccctc agagccgcca ccagaaccac caccagagcc gccgccagca
    13381 ttgacaggag gcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
    13441 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
    13501 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
    13561 attcaacaga aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa
    13621 actttattgc caaatgtttg aacgatcggg gatcatccgg gtctgtggcg ggaactccac
    13681 gaaaatatcc gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc
    13741 gccgacgccg ttgatgtgga cgccgggccc gatcatattg tcgctcagga tcgtggcgtt
    13801 gtgcttgtcg gccgttgctg tcgtaatgat atcggcacct tcgaccgcct gttccgcaga
    13861 gatcccgtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
    13921 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
    13981 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
    14041 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
    14101 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
    14161 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
    14221 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
    14281 agatcatcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
    14341 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
    14401 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
    14461 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
    14521 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
    14581 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
    14641 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
    14701 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
    14761 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
    14821 tgttcaatca tgcgaaacga tccagatccg gtgcagatta tttggattga gagtgaatat
    14881 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag
    14941 aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg
    15001 acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt
    15061 gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa
    15121 cgtgactccc ttaattctcc gctcatgatc agattgtcgt ttcccgcctt cagtttaaac
    15181 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
    15241 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
    15301 gccaaccaca gggttcccca gatctggcgc cggccagcga gacgagcaag attggccgcc
    15361 gcccgaaacg atccgacagc gcgcccagca caggtgcgca ggcaaattgc accaacgcat
    15421 acagcgccag cagaatgcca tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca
    15481 ggaggcccgg cagcaccggc ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc
    15541 tcagaattac gatcaggggt atgttgggtt tcacgtctgg cctccggacc agcctccgct
    15601 ggtccga
//
