LOCUS       sequence-11553-e        9900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 9900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pT7-EGFP-C1-HsTNRC6A
FEATURES             Location/Qualifiers
     source          1..9900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    5617..5628
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6688..6809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6725..6744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6779..6798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6816..7271)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6953..6974)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7165..7184
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7298..7402
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7404..7761
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7404..7595
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7427..7447)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7612..7747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7796..8590
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7796..8590
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7850..7869)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8460..8479
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8778..8797)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8822..8869
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9198..9786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9687..9706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctatggatgc tgattctgcc tccagttctg aatcagagag
     1441 aaacatcact atcatggctt cagggaacac aggtggtgaa aaagatggcc ttcggaatag
     1501 cactggactt ggttcccaaa acaagtttgt agttggtagc agcagcaata atgtgggcca
     1561 tggaagtagt actgggccat ggggtttttc ccatggagcc ataataagca catgtcaggt
     1621 ctctgtggat gctcctgaaa gcaaatctga aagtagcaac aatagaatga atgcttgggg
     1681 cactgtaagt tcttcatcaa atggagggtt aaatccaagc actttgaatt cagctagcaa
     1741 ccatggtgcc tggccagtat tagagaacaa tggacttgcc ctaaaagggc ctgtagggag
     1801 tggtagttct ggcattaata ttcagtgcag tactataggc cagatgccta acaatcagag
     1861 tattaactct aaagtgagtg gtggttctac ccatggtacc tggggaagcc ttcaggaaac
     1921 ttgtgaatct gaagtaagtg gtacacagaa ggtttcattc agtggtcaac ctcaaaatat
     1981 taccactgaa atgactggac caaataacac tactaacttt atgacctcta gtttaccaaa
     2041 ctccggttca gtgcagaata atgagctgcc tagtagtaac acaggggcct ggcgtgtgag
     2101 cacaatgaat catcctcaga tgcaggctcc atcaggtatg aatggcactt ccctttctca
     2161 ccttagcaat ggagagtcaa aaagtggagg ctcttatggt actacatggg gtgcctatgg
     2221 ttctaattac tctggagaca aatgttcagg ccctaatggc caagctaatg gtgacactgt
     2281 gaatgcaact ctaatgcagc ctggcgtaaa tggtcctatg ggcactaact ttcaagttaa
     2341 cacaaacaaa ggaggtggtg tgtgggaatc tggtgcagca aactcccaga gtacatcatg
     2401 gggaagtgga aatggcgcaa attctggagg aagtcgaaga ggatggggaa cccctgcaca
     2461 aaacactggc actaatttac ccagcgttga gtggaacaaa ctgcctagca atcagcattc
     2521 caatgatagt gcaaatggca atggtaagac gtttacaaat ggatggaaat ctactgagga
     2581 agaggatcag ggttctgcca catctcagac aaatgagcaa agcagtgtgt gggccaaaac
     2641 aggaggtaca gtggagagcg atggtagtac agaaagcact ggacgccttg aggaaaaagg
     2701 aactggggaa agtcagagta gagacagaag aaaaattgat cagcacacat tactccaaag
     2761 cattgtaaac agaactgact tagatccacg tgtcctgtcc aactctggtt ggggacagac
     2821 tcctattaag cagaatactg cctgggatac agaaacatca cctagagggg aacgaaagac
     2881 tgacaatggg acagaggcct ggggaagctc tgcaacacag acttttaact caggggcatg
     2941 tatagataag actagcccta atggtaatga tacctcatct gtatcagggt ggggcgatcc
     3001 caaacctgct ctgaggtggg gagattccaa aggctcaaac tgccaggggg ggtgggaaga
     3061 tgattctgct gctacaggaa tggtcaagag caatcagtgg gggaattgca aagaggagaa
     3121 ggctgcatgg aatgactcgc aaaagaataa acagggatgg ggtgatggac aaaaatcaag
     3181 ccaagggtgg tctgtttctg ccagtgataa ctggggagaa acttcaagga ataaccattg
     3241 gggtgaggcc aataagaaat ccagctcagg aggtagtgac agtgacaggt ccgtttccgg
     3301 ttggaacgaa cttggtaaaa ctagttcttt cacttgggga aacaacataa atccaaataa
     3361 ttcatcagga tgggatgaat cttctaaacc tactccttcc cagggatggg gagaccctcc
     3421 aaagtctaat cagtctctag gttggggaga ttcgtcaaag ccagtcagct ctccagactg
     3481 gaacaagcaa caagacattg ttggatcttg gggaatccca ccagctacag gcaaacctcc
     3541 tggtacaggc tggctggggg gacctatacc agccccagca aaagaagaag aacccacagg
     3601 ctgggaggaa ccatccccag aatctatacg tcgcaaaatg gagattgatg atggaacttc
     3661 agcttgggga gatccaagca aatacaacta caaaaatgtg aacatgtgga acaaaaacgt
     3721 cccaaatggc aacagccgtt cagaccagca agcacaggta catcagctgc taacgcctgc
     3781 aagtgccatc tcaaacaaag aggcaagcag tggctctggc tggggtgagc cctgggggga
     3841 gccttctact ccagccacaa ctgtggataa tggtacttca gcatggggta agcccataga
     3901 cagtggtccc agctgggggg aacccattgc tgcggcatcc agcacatcca cgtggggctc
     3961 cagctctgtt ggtccacaag cattaagcaa atctgggcca aaatctatgc aagatggctg
     4021 gtgtggtgat gatatgccat tgcctggaaa tcgccccact ggctgggaag aggaagagga
     4081 tgtggagatt ggaatgtgga atagtaattc atctcaagag cttaactcat ctttaaattg
     4141 gccaccatat acaaagaaaa tgtcatcgaa gggtctgagt ggcaaaaaaa ggagaaggga
     4201 aaggggaatg atgaaaggtg gaaacaaaca agaagaagcg tggataaatc catttgttaa
     4261 acagttttca aacatcagtt tttcgagaga ctcaccagag gaaaatgtac aaagcaataa
     4321 gatggacctt tctggaggaa tgttacaaga caaacgaatg gagatagata aacatagcct
     4381 aaatattggt gattacaatc gaacggtcgg gaaaggccct ggttctcggc ctcagatttc
     4441 caaagagtct tccatggagc gcaatcctta ttttgataag gatggcattg tagcagatga
     4501 atcccaaaac atgcagttta tgtccagtca aagcatgaag cttccccctt caaatagtgc
     4561 actacctaac caggcccttg gctccatagc agggctgggt atgcaaaact tgaattctgt
     4621 tagacagaat ggcaatccca gtatgtttgg tgttggaaac acagcagcac aaccccgggg
     4681 catgcagcag cctccagcac aacctcttag ttcatctcag cctaatctcc gtgctcaagt
     4741 gcctcctcca ttactctccc ctcaggttcc agtttcattg ctgaagtatg caccaaacaa
     4801 cggtggcctg aatccactct ttggccctca acaggtagcc atgctgaacc agctatccca
     4861 gctaaaccag ctttctcaga tctcccagtt acagcgattg ttagcgcagc agcaaagggc
     4921 gcagagtcag agaagcgtgc cttctgggaa ccggccgcag caagaccagc agggtcgacc
     4981 tcttagtgtg cagcagcaaa tgatgcaaca atctcgtcaa cttgatccaa acctgttggt
     5041 gaagcagcag actccaccat ctcagcagca gccactccat cagccagcca tgaagtcttt
     5101 ccttgacaat gtcatgcccc acactacacc tgagctgcaa aaagggccat caccaataaa
     5161 tgctttcagc aacttcccta taggcttgaa ctcaaacttg aatgtaaata tggatatgaa
     5221 cagtattaaa gagccacagt caagactaag gaagtggacg acagtggaca gcatttctgt
     5281 gaacacatct ttggatcaaa actccagcaa acatggtgct atttcaagtg gtttcaggct
     5341 ggaagagtct ccatttgttc cctatgactt tatgaacagc agtacttcac cagccagtcc
     5401 tccaggttca ataggagatg gctggccacg tgccaaatcg cctaacggct ctagcagtgt
     5461 taattggcca ccagaatttc gtcctggtga gccatggaaa ggttatccaa acattgaccc
     5521 tgaaactgac ccttacgtca ctcctggcag tgtcataaac aatctttcaa ttaatactgt
     5581 gcgggaagtt gaccacctca gggacaggaa cagtgggtca tcctcatcct tgaacaccac
     5641 gctgccttca actagtgcct ggtcatccat tcgtgcctcc aactacaacg ttcccctcag
     5701 cagtacagca caaagcactt cagccagaaa tagtgattcc aaattgacat ggtctcctgg
     5761 ttcagttaca aacacctctc tggctcatga gctgtggaag gtccctttgc cacctaaaaa
     5821 catcactgct ccgtcccgcc cacctccggg actgactggt cagaagccac ccttgtctac
     5881 gtgggataat tctccccttc gtataggtgg aggatgggga aattctgacg ccagatatac
     5941 cccaggttcc agctggggtg agagcagctc agggagaata acaaattggc ttgttctaaa
     6001 aaaccttaca cctcagatcg atggctcaac tctgcgcact ctgtgcatgc agcacggccc
     6061 gctgatcaca ttccacctga acctccctca cggaaatgct ctggtccgct acagttcaaa
     6121 agaagaggta gtgaaggcac aaaagtctct gcacatgtgt gtactgggga acactactat
     6181 tcttgctgag tttgccagtg aagaggagat cagtcgtttc tttgcacaaa gccagtctct
     6241 gaccccttct cccggctggc agtctctcgg gtccagccag agccggctgg gctccctcga
     6301 ctgttcccac tcattctcca gccggaccga tctcaatcac tggaatggtg ctgggctgtc
     6361 gggaactaac tgtggagacc ttcacggcac ttcactctgg gggaccccgc attattccac
     6421 aagcctgtgg ggtcccccaa gcagcagcga cccccgagga attagcagcc catctcccat
     6481 taacgctttt ctttctgttg accacctggg tgggggtgga gagtccatgt aactgcagtc
     6541 gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc
     6601 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     6661 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     6721 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6781 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
     6841 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     6901 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     6961 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     7021 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     7081 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     7141 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     7201 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     7261 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7321 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7381 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     7441 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7501 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7561 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7621 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7681 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7741 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     7801 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     7861 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     7921 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     7981 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     8041 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     8101 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     8161 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     8221 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     8281 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     8341 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     8401 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     8461 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     8521 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     8581 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     8641 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     8701 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     8761 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     8821 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     8881 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     8941 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     9001 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     9061 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9121 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9181 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9241 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9301 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     9361 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9421 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9481 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9541 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9601 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9661 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9721 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9781 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9841 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
