LOCUS       sequence-115531-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary T-DNA vector expressing EGFP and neomycin phosphotransferase 
            II (NPTII); for Agrobacterium tumefaciens-mediated transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Maximova S, Miller C, Antunez de Mayolo G, Pishak S, Young A, 
            Guiltinan MJ
  TITLE     Stable transformation of Theobroma cacao L. and influence of matrix 
            attachment regions on GFP expression.
  JOURNAL   Plant Cell Rep. 2003 Jun;21(9):872-83. Epub 2003 Apr 3.
  PUBMED    12789505
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2003-06"; volume: "21(9)"; pages: "872-83. Epub 2003 
            Apr 3"
COMMENT     Sequence Label: pGH00.0126
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    78..252
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(283..300)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(283..299)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(292..314)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(439..458)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     649..670
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagaat
       61 tggcatgcaa gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt
      121 agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
      181 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact
      241 aaaatccaga tcccccgaat tccgggtacc gagctcgaat tcactggccg tcgttttaca
      301 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      361 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      421 cagcctgaat ggcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      481 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
      541 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
      601 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
      661 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
      721 tgccgatttc ggaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
      781 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
      841 actggtgaa
//
