LOCUS       sequence-115535-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytoplasmic ER hook only (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Str-Ii_neomycin
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             642..668
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcga tatcatgcac cggaggagat
      121 cacgctcttg tagggaggac cagaaacctg tcaccggtga ccctagcaaa gactcaaaag
      181 ctcaggtgtc cgctgccgag gctggcatta ctggaacatg gtacaatcag ctcgggagca
      241 cctttattgt gactgctgga gccgatggag ccctcaccgg aacatacgaa tctgctgtgg
      301 gaaacgccga atcacggtac gtcctcactg gccgatacga tagtgcccct gccaccgacg
      361 gatctgggac tgccctggga tggactgtcg cttggaaaaa caactaccgg aatgctcatt
      421 ctgccacaac atggagtgga cagtacgtgg gaggcgctga ggctagaatc aatacacagt
      481 ggctgctcac atctggcaca accgaggcaa atgcttggaa atccaccctg gtgggacatg
      541 acacattcac caaagtgaaa ccctccgccg cttcaatcga tgccgccaaa aaagccggag
      601 tcaacaacgg caatcctctg gatgccgtcc agcaggtcga ctatccgtac gacgtaccag
      661 actacgcagt cggaccgatg gacgatcaga gggacctcat tagcaacaac gaacagctgc
      721 ctatgctggg acggcgacct ggagcccctg aatccaaatg ctcta
//
