LOCUS       sequence-115553-       13104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens 
            (B. japonicum) scoI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM
  TITLE     Stable fluorescent and enzymatic tagging of Bradyrhizobium 
            diazoefficiens to analyze host-plant infection and colonization.
  JOURNAL   Mol Plant Microbe Interact. 2015 Jun 2.
  PUBMED    26035130
REFERENCE   2  (bases 1 to 13104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2015 Jun 2."
COMMENT     Sequence Label: pRJPaph-lacZYA
FEATURES             Location/Qualifiers
     source          1..13104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            1271..1380
                     /note="incP origin of transfer"
     CDS             1413..1784
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     protein_bind    3440..3456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3445..3467
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3464..3480
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3464..3480
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3476..6550
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMIQACDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(3505..3527)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10010..10598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10499..10518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10752..10769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10784..10924
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10850..10869)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11010..11032
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(11026..11217)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(11858..13048)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     promoter        complement(join(13096..13101,13102..13104,1..14,15..20))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 tatcgatgat aagctgtcaa acatggcctg tcgcttgcgg tattcggaat cttgcacgcc
       61 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
      121 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
      181 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
      241 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcttcc
      301 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
      361 gccacgatgc gtccggcgta gaggatcacc acgtcatcct tggtcggcgc aatcagctcg
      421 accagggtgt cgaagttgcg cgagttaatt tcgtcgccgg ccatgatgtt cagccggcgg
      481 acgttcaggg ccttgtagcc ctcgaacgtc gcgttcaccg ggtcggtgtc gatgcacaag
      541 ggtgtctgcc ccttgtccat cttgtactgc gcaatgatcg cggcgatggc cgacttgccg
      601 accccgccct tgccctgcaa aaccatgtga attttcgcca ttacagtaga tcctttttgt
      661 ccggtgttgg gttgaaggtg aagccggtcg gggccgcagc gggggccggc ttttcagcct
      721 tgcccccctg cttcggccgc cgtggctccg gcgtcttggg tgccggcgcg ggttccgcag
      781 ccttggcctg cggtgcgggc acatcggcgg gcttggcctt gatgtgccgc ctggcgtgcg
      841 agcggaacgt ctcgtaggag aacttgacct tccccgtttc ccgcatgtgc tcccaaatgg
      901 tgacgagcgc atagccggac gctaacgccg cctcgacatc cgccctcacc gccaggaacg
      961 caaccgcagc ctcatcacgc cggcgcttct tggccgcgcg ggattcaacc cactcggcca
     1021 gctcgtcggt gtagctcttt ggcatcgtct ctcgcctgtc ccctcagttc agtaatttcc
     1081 tgcatttgcc tgtttccagt cggtagatat tccacaaaac agcagggaag cagcgctttt
     1141 ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt
     1201 tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac
     1261 ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca
     1321 ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgg
     1381 ctaccgccgg cgtaacagat gagggcaagc ggatggctga tgaaaccaag ccaaccagga
     1441 agggcagccc acctatcaag gtgtactgcc ttccagacga acgaagagcg attgaggaaa
     1501 aggcggcggc ggccggcatg agcctgtcgg cctacctgct ggccgtcggc cagggctaca
     1561 aaatcacggg cgtcgtggac tatgagcacg tccgcgagct ggcccgcatc aatggcgacc
     1621 tgggccgcct gggcggcctg ctgaaactct ggctcaccga cgacccgcgc acggcgcggt
     1681 tcggtgatgc cacgatcctc gccctgctgg cgaagatcga agagaagcag gacgagcttg
     1741 gcaaggtcat gatgggcgtg gtccgcccga gggcagagcc atgacttttt tagccgctaa
     1801 aacggccggg gggtgcgcgt gattgccaag cacgtcccca tgcgctccat caagaagagc
     1861 gacttcgcgg agctggtgaa gtacatcacc gacgagcaag gcaagaccga gcgcctgggt
     1921 cacgtgcgcg tcacgaactg cgaggcaaac accctgcccg ctgtcatggc cgaggtgatg
     1981 gcgacccagc acggcaacac ccgttccgag gccgacaaga cctatcacct gctggttagc
     2041 ttccgcgcgg gagagaagcc cgacgcggag acgttgcgcg cgattgagga ccgcatctgc
     2101 gctgggcttg gcttcgccga gcatcagcgc gtcagtgccg tgcatcacga caccgacaac
     2161 ctgcacatcc atatcgccat caacaagatt cacccgaccc gaaacaccat ccatgagccg
     2221 tatcgggcct accgcgccct cgctgacctc tgcgcgacgc tcgaacggga ctacgggctt
     2281 gagcgtgaca atcacgaaac gcggcagcgc gtttccgaga accgcgcgaa cgacatggag
     2341 cggcacgcgg gcgtggaaag cctggtcggc tggatcatat cgtcaattat tacctccacg
     2401 gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac actgcttccg
     2461 gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct
     2521 gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac
     2581 ttattcaggc gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg
     2641 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     2701 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     2761 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     2821 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
     2881 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     2941 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     3001 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     3061 ctttcattgc catacggaat tcgagctcgc acgctgccgc aagcactcag ggcgcaaggg
     3121 ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat
     3181 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt
     3241 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga
     3301 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg
     3361 gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctg atcaagagac
     3421 aggatactag tgtgcagaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     3481 catgattcaa gcttgcgatc ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3541 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3601 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc
     3661 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc
     3721 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac
     3781 caacgtaacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg
     3841 ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat
     3901 tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta
     3961 cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga
     4021 aaaccgcctc gcggtgatgg tgctgcgttg gagtgacggc agttatctgg aagatcagga
     4081 tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca
     4141 aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga
     4201 ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg
     4261 gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga
     4321 gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg
     4381 gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac
     4441 gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct
     4501 gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc
     4561 tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca
     4621 gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct
     4681 gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt
     4741 gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac
     4801 gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga
     4861 atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc
     4921 ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc
     4981 gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa
     5041 aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc
     5101 gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg
     5161 tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa
     5221 cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt
     5281 ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa
     5341 acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga
     5401 atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa
     5461 gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga
     5521 actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt
     5581 gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg
     5641 tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac
     5701 caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca
     5761 gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg
     5821 cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat
     5881 tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc
     5941 gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc
     6001 gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag
     6061 tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg
     6121 gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg
     6181 gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc
     6241 attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac
     6301 gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg
     6361 ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg
     6421 cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc
     6481 gtcagtatcg gcggaattac agctgagcgc cggtcgctac cattaccagt tggtctggtg
     6541 tcaaaaataa taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat
     6601 tatgtactat ttaaaaaaca caaacttttg gatgttcggt ttattctttt tcttttactt
     6661 ttttatcatg ggagcctact tcccgttttt cccgatttgg ctacatgaca tcaaccatat
     6721 cagcaaaagt gatacgggta ttatttttgc cgctatttct ctgttctcgc tattattcca
     6781 accgctgttt ggtctgcttt ctgacaaact cgggctgcgc aaatacctgc tgtggattat
     6841 taccggcatg ttagtgatgt ttgcgccgtt ctttattttt atcttcgggc cactgttaca
     6901 atacaacatt ttagtaggat cgattgttgg tggtatttat ctaggctttt gttttaacgc
     6961 cggtgcgcca gcagtagagg catttattga gaaagtcagc cgtcgcagta atttcgaatt
     7021 tggtcgcgcg cggatgtttg gctgtgttgg ctgggcgctg tgtgcctcga ttgtcggcat
     7081 catgttcacc atcaataatc agtttgtttt ctggctgggc tctggctgtg cactcatcct
     7141 cgccgtttta ctctttttcg ccaaaacgga tgcgccctct tctgccacgg ttgccaatgc
     7201 ggtaggtgcc aaccattcgg catttagcct taagctggca ctggaactgt tcagacagcc
     7261 aaaactgtgg tttttgtcac tgtatgttat tggcgtttcc tgcacctacg atgtttttga
     7321 ccaacagttt gctaatttct ttacttcgtt ctttgctacc ggtgaacagg gtacgcgggt
     7381 atttggctac gtaacgacaa tgggcgaatt acttaacgcc tcgattatgt tctttgcgcc
     7441 actgatcatt aatcgcatcg gtgggaaaaa cgccctgctg ctggctggca ctattatgtc
     7501 tgtacgtatt attggctcat cgttcgccac ctcagcgctg gaagtggtta ttctgaaaac
     7561 gctgcatatg tttgaagtac cgttcctgct ggtgggctgc tttaaatata ttaccagcca
     7621 gtttgaagtg cgtttttcag cgacgattta tctggtctgt ttctgcttct ttaagcaact
     7681 ggcgatgatt tttatgtctg tactggcggg caatatgtat gaaagcatcg gtttccaggg
     7741 cgcttatctg gtgctgggtc tggtggcgct gggcttcacc ttaatttccg tgttcacgct
     7801 tagcggcccc ggcccgcttt ccctgctgcg tcgtcaggtg aatgaagtcg cttaagcaat
     7861 caatgtcgga tgcggcgcga cgcttatccg accaacatat cataacggag tgatcgcatt
     7921 gaacatgcca atgaccgaaa gaataagagc aggcaagcta tttaccgata tgtgcgaagg
     7981 cttaccggaa aaaagacttc gtgggaaaac gttaatgtat gagtttaatc actcgcatcc
     8041 atcagaagtt gaaaaaagag aaagcctgat taaagaaatg tttgccacgg taggggaaaa
     8101 cgcctgggta gaaccgcctg tctatttctc ttacggttcc aacatccata taggccgcaa
     8161 tttttatgca aatttcaatt taaccattgt cgatgactac acggtaacaa tcggtgataa
     8221 cgtactgatt gcacccaacg ttactctttc cgttacggga caccctgtac accatgaatt
     8281 gagaaaaaac ggcgagatgt actcttttcc gataacgatt ggcaataacg tctggatcgg
     8341 aagtcatgtg gttattaatc caggcgtcac catcggggat aattctgtta ttggcgcggg
     8401 tagtatcgtc acaaaagaca ttccaccaaa cgtcgtggcg gctggcgttc cttgtcgggt
     8461 tattcgcgaa ataaacgacc gggataagca ctattatttc aaagattata aagttgaatc
     8521 gtcagtttaa attataaaaa ttgcctgata cgctgcgctt atcagggaac tgcaggcgtt
     8581 atctgtagtt tggtggatcg gtcggaccgc gtcctcggtg taacctctcc cgcttgcggg
     8641 agaggtatag gccgcttttg gcggccgtcc tctaagacgc cgaggcaaag cctcggctat
     8701 ggcagagcgc cgggtgaggg ctctctcctc tcggggattg tccctccgcg gagacaccct
     8761 ctccccgccc ccctcccgca agcgggagag ggagcgcacc ttcaccctga ccgcaatcgg
     8821 cgctcgcgtt cccgggcgga tggtctataa ggccctccga tcccaacgaa attcgttcat
     8881 ttccggccga tggctccatc gcaacaggcg aaatcgtcca aatctgcccg tggcttgctg
     8941 acagggctgg ccgtcggcgc ctgcctgttc gccggcttgc ccggcgcgca ggcgcaggcc
     9001 ccggccaagc agcctccggc ggcgccccag gtcaccccgc aggtcgcgcc ccaggcggcc
     9061 ccgcaggccg tgcccggctt ctgggacccg cggcggcggc cggagcggcc ggacctgtcg
     9121 cgcctgaccg tgatccgctt tttgaccgag accgactatc cgccgttcaa ctacaccggc
     9181 gccgacggca atccggccgg cttcaacgtc gatctcgccc gcgcgctctg cgaggagatc
     9241 aaggtcacct gcacggtgca gatgcgccgc ttcgagaccc tggtcgacgc gctcacctcg
     9301 aaccggggcg atgctatcat cgcctcgatg gcggtgagcc cgcagctccg ggcccgcgtc
     9361 gacttctccg atccctatta ccgtctagag cggccgccac cgcggtggaa aaagtcatgg
     9421 ctctgccctc gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc
     9481 gatcttcgcc agcagggcga ggatcgtggc atcaccgaac cgcggcaatg gcaacaacgt
     9541 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9601 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9661 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9721 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9781 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9841 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9901 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9961 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10021 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10081 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10141 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10201 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10261 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10321 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10381 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10441 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10501 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10561 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10621 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10681 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10741 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
    10801 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
    10861 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
    10921 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
    10981 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
    11041 gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga
    11101 ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa
    11161 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcacttg
    11221 atgcctccgt gtaaggggga atttctgttc atgggggtaa tgataccgat gaaacgagag
    11281 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag
    11341 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc
    11401 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg
    11461 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca
    11521 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg
    11581 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag
    11641 cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg
    11701 ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc
    11761 caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga
    11821 gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc
    11881 atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg
    11941 ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag
    12001 tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt
    12061 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
    12121 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
    12181 ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
    12241 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
    12301 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
    12361 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
    12421 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
    12481 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
    12541 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
    12601 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
    12661 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
    12721 cggtgatgcc ggccacgatg cgtccggcgt agaggatcca caggacgggt gtggtcgcca
    12781 tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa
    12841 agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg
    12901 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc
    12961 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga
    13021 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact
    13081 gtgataaact accgcattaa agct
//
