LOCUS       sequence-115554-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synchronize trafficking of Ecadherin from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Ii-Str_SBP-mCherry-Ecadherin
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     153..212
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             213..323
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(478..496)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     746..765
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
       61 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
      121 tgataagctt gccacaaccc gggaggcgcg ccatgtacag gatgcaactc ctgtcttgca
      181 ttgcactaag tcttgcactt gtcacgaatt ccgacgagaa gaccactggt tggcgaggtg
      241 gacacgttgt tgaaggactg gctggggaac ttgaacaact tcgtgcacga ctggagcatc
      301 acccacaagg tcaacgtgaa ccacctgcag gtatggtgag caagggcgag gaggataaca
      361 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
      421 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      481 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      541 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      601 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      661 tgaccgtgac ccaggactcc tccctacagg acggcgagtt catctacaag gtgaagctgc
      721 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      781 cctcctccga gcggatgta
//
