LOCUS       sequence-115558-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ER calcium sensor for lentivirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Henderson MJ, Baldwin HA, Werley CA, Boccardo S, Whitaker LR, Yan X,
            Holt GT, Schreiter ER, Looger LL, Cohen AE, Kim DS, Harvey BK
  TITLE     A Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic 
            Reticulum Calcium Store.
  JOURNAL   PLoS One. 2015 Oct 9;10(10):e0139273. doi: 
            10.1371/journal.pone.0139273. eCollection 2015.
  PUBMED    26451944
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-9"; volume: "10(10):e0139273. doi"; pages: " 
            10.1371/journal.pone.0139273. eCollection 2015"
COMMENT     Sequence Label: pMOS003-lenti-CMV-GCaMPer
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..115
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             119..151
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(155..163,164..178)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      185..194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             206..262
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     475..496
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(602..623)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(863..882)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctccatagaa gacaccgact ctagaggatc cgccaccatg ctgctatccg tgccgttgct
       61 gctcggcctc ctcggcctgg ccgtcgccat gggttctcat catcatcatc atcatggtat
      121 ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga
      181 tctcgccacc atggtcgact catcacgtcg taagtggaat aagacaggtc acgcagtcag
      241 agctataggt cggctgagct cactcgagaa cgtctatatc aaggccgaca agcagaagaa
      301 cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
      361 ctaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      421 ctacctgagc gtgcagtcca aactttcgaa agaccccaac gagaagcgcg atcacatggt
      481 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg
      541 cggtaccgga gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      601 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga
      661 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      721 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
      781 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacatcca
      841 ggagcgcacc atcttcttca aggacgacgg caactacaag acc
//
