LOCUS       sequence-115563-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 4-6 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX2-62
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(391..408)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(391..407)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(400..422)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      471..557
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      649..676
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgcgtcacc cgggcaacct tgggtagcag cgaagtcgag gcatttctgt cctggctggt
       61 ctagacgtct cgccatggtc ctggcacagc accggcaaca gccgctgcac cgtttcgagc
      121 gcttgcttgc cgccatcgtg gctggcgata gccaccactt ggtccggagt caggccatgg
      181 tcctggcaca gcaccggcaa cagccgctgc accgtttcga gcgcttgctt gccgccaccg
      241 ttgctggcga tagccaccac ttggtccggg gtcaggccat ggtcctggca cagcaccggc
      301 aacagccgct gcaccgtttc gagcgcttgc ttgccgccac cgttgctggc gatagccacc
      361 acttggtccg gggtcaggga gacgcttaag actggccgtc gttttacaac gtcgtgactg
      421 ggaaaacatc catgctagcg ttaacgcgag agtagggaac tgccaggcat caaataaaac
      481 gaaaggctca gtcggaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      541 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag
      601 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aactaagcag aaggccatcc
      661 tgacggatgg cctttttgcg tttctacaaa
//
