LOCUS       sequence-115575-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 4-6 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX2-32
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(389..406)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(389..405)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(398..420)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      469..555
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      647..674
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc atttctgtcc tggctggtct
       61 agacgtctcg ccatggtcct ggcacagcac cggcaacagc cgctgcaccg tttcgagcgc
      121 ttgcttgccg ccaccgttgc tggcgatagc caccacttgg tccggggtca ggccatggtc
      181 ctggcacagc accggcaaca gccgctgcac cgtttcgagc gcttgcttgc cgccaccgtt
      241 gctggcgata gccaccactt ggtccggggt caggccatgg tcctggcaca gcaccggcaa
      301 cagccgctgc accgtttcga gcgcttgctt gccgccatcg tggctggcga tagccaccac
      361 ttggtccggg gtcagggaga cgcttaagac tggccgtcgt tttacaacgt cgtgactggg
      421 aaaacatcca tgctagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga
      481 aaggctcagt cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      541 ctgagtagga caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccggaggg
      601 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg
      661 acggatggcc tttttgcgtt tctacaaact cttcctggct agcggtacgc gtattaatt
//
