LOCUS       sequence-115578-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 10-12 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX4-1
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(394..411)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(394..410)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(403..425)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      474..560
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      652..679
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagacgt ctcgccatgg tcctggcaca gcaccggcaa cagccgctgc accgtttcga
      121 gcgcttgctt gccgccaatg ttgctggcga tagccaccac ttggtccggg gtcaggccat
      181 ggtcctggca cagcaccggc aacagccgct gcaccgtttc gagcgcttgc ttgccgccaa
      241 tgttgctggc gatagccacc acttggtccg gggtcaggcc atggtcctgg cacagcaccg
      301 gcaacagccg ctgcaccgtt tcgagcgctt gcttgccgcc aatgttgctg gcgatagcca
      361 ccacttggtc cggggtcagg ggagacgctt aagactggcc gtcgttttac aacgtcgtga
      421 ctgggaaaac atccatgcta gcgttaacgc gagagtaggg aactgccagg catcaaataa
      481 aacgaaaggc tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      541 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg
      601 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaactaag cagaaggcca
      661 tcctgacgga tggccttttt gcgtttctac aaactcttcc tggctagcgg tacgcgtatt
      721 aattgcgttg cgctcactgc ccgctttcca gtc
//
