LOCUS       sequence-115587-        5176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  G protein gamma-11 tagged with HaloTag on amino terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5176)
  TITLE     Berlot Lab HaloTag Fusions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HaloTag-G protein gamma-11
FEATURES             Location/Qualifiers
     source          1..5176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1075..1084
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2285..2406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2296..2315)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2350..2369
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2592..3047
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2679..2698)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2889..2910
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3114..3133)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3233..3255
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3293..3311)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3379..3483
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3484..3552,3553..4344)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3702..3721)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4515..5103
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5004..5023
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagccag ctggcgcgga tatcgccacc
     1081 atgggatccg aaatcggtac aggcttcccc ttcgaccccc attatgtgga agtcctgggc
     1141 gagcgtatgc actacgtcga tgttggaccg cgggatggca cgcctgtgct gttcctgcac
     1201 ggtaacccga cctcgtccta cctgtggcgc aacatcatcc cgcatgtagc accgagtcat
     1261 cggtgcattg ctccagacct gatcgggatg ggaaaatcgg acaaaccaga cctcgattat
     1321 ttcttcgacg accacgtccg ctacctcgat gccttcatcg aagccttggg tttggaagag
     1381 gtcgtcctgg tcatccacga ctggggctca gctctcggat tccactgggc caagcgcaat
     1441 ccggaacggg tcaaaggtat tgcatgtatg gaattcatcc ggcctatccc gacgtgggac
     1501 gaatggccag aattcgcccg tgagaccttc caggccttcc ggaccgccga cgtcggccga
     1561 gagttgatca tcgatcagaa cgctttcatc gagggtgcgc tcccgatggg ggtcgtccgt
     1621 ccgcttacgg aggtcgagat ggaccactat cgcgagccct tcctcaagcc tgttgaccga
     1681 gagccactgt ggcgattccc caacgagctg cccatcgccg gtgagcccgc gaacatcgtc
     1741 gcgctcgtcg aggcatacat gaactggctg caccagtcac ctgtcccgaa gttgttgttc
     1801 tggggcacac ccggcgtact gatccccccg gccgaagccg cgagacttgc cgaaagcctc
     1861 cccaactgca agacagtgga catcggcccg ggattgttct tgctccagga agacaacccg
     1921 gaccttatcg gcagtgagat cgcgcgctgg ctccccgggc tggccggctc cggtgggggc
     1981 gggagcatgc ctgcccttca catcgaagat ttgccagaga aggaaaaact gaaaatggaa
     2041 gttgagcagc ttcgcaaaga agtgaagttg cagagacaac aagtgtctaa atgttctgaa
     2101 gaaataaaga actatattga agaacgttct ggagaggacc ctctagtaaa gggaattcca
     2161 gaagacaaga acccctttaa agaaaaaggc agctgtgtta tttcataagg atccgccggc
     2221 taatagttaa ttaagtaggc ggccgcttcc ctttagtgag ggttaatgct tcgagcagac
     2281 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     2341 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     2401 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     2461 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tccgataagg atcgatccgg
     2521 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     2581 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2641 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2701 ctttctcgcc acgttcgcgg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2761 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2821 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2881 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2941 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     3001 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat
     3061 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     3121 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     3181 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     3241 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     3301 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     3361 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3421 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3481 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     3541 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     3601 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     3661 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     3721 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     3781 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     3841 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     3901 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     3961 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4021 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4081 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     4141 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     4201 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     4261 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     4321 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     4381 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     4441 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     4501 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4561 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4621 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     4681 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4741 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4801 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4861 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4921 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4981 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5041 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5101 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5161 atggctcgac agatct
//
