LOCUS       sequence-115589-        5213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  G protein gamma-12 tagged with HaloTag on amino terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5213)
  TITLE     Berlot Lab HaloTag Fusions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HaloTag-G protein gamma-12
FEATURES             Location/Qualifiers
     source          1..5213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1075..1084
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2322..2443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2333..2352)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2387..2406
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2629..3084
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2716..2735)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2926..2947
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3151..3170)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3270..3292
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3330..3348)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3416..3520
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3521..3589,3590..4381)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3739..3758)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4552..5140
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5041..5060
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagccag ctggcgcgga tatcgccacc
     1081 atgggatccg aaatcggtac aggcttcccc ttcgaccccc attatgtgga agtcctgggc
     1141 gagcgtatgc actacgtcga tgttggaccg cgggatggca cgcctgtgct gttcctgcac
     1201 ggtaacccga cctcgtccta cctgtggcgc aacatcatcc cgcatgtagc accgagtcat
     1261 cggtgcattg ctccagacct gatcgggatg ggaaaatcgg acaaaccaga cctcgattat
     1321 ttcttcgacg accacgtccg ctacctcgat gccttcatcg aagccttggg tttggaagag
     1381 gtcgtcctgg tcatccacga ctggggctca gctctcggat tccactgggc caagcgcaat
     1441 ccggaacggg tcaaaggtat tgcatgtatg gaattcatcc ggcctatccc gacgtgggac
     1501 gaatggccag aattcgcccg tgagaccttc caggccttcc ggaccgccga cgtcggccga
     1561 gagttgatca tcgatcagaa cgctttcatc gagggtgcgc tcccgatggg ggtcgtccgt
     1621 ccgcttacgg aggtcgagat ggaccactat cgcgagccct tcctcaagcc tgttgaccga
     1681 gagccactgt ggcgattccc caacgagctg cccatcgccg gtgagcccgc gaacatcgtc
     1741 gcgctcgtcg aggcatacat gaactggctg caccagtcac ctgtcccgaa gttgttgttc
     1801 tggggcacac ccggcgtact gatccccccg gccgaagccg cgagacttgc cgaaagcctc
     1861 cccaactgca agacagtgga catcggcccg ggattgttct tgctccagga agacaacccg
     1921 gaccttatcg gcagtgagat cgcgcgctgg ctccccgggc tggccggctc cggtgggggc
     1981 gggagcatgt ccagcaaaac agcaagcacc aacaatatag cccaggcaag gagaactgtg
     2041 cagcagttaa gattagaagc ctccattgaa agaataaagg tttcgaaggc atcagcggac
     2101 ctcatgtcct actgtgagga acatgccagg agtgaccctt tgctgatagg aataccaact
     2161 tcagaaaacc ctttcaagga taaaaaaact tgcatcatct tatagtggaa tagagaaaca
     2221 gctcctcgcc tcttcccaac aacgcggatc cgccggctaa tagttaatta agtaggcggc
     2281 cgcttccctt tagtgagggt taatgcttcg agcagacatg ataagataca ttgatgagtt
     2341 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2401 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2461 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     2521 ctacaaatgt ggtaaaatcc gataaggatc gatccgggct ggcgtaatag cgaagaggcc
     2581 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
     2641 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     2701 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgcgggct
     2761 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     2821 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     2881 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     2941 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3001 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3061 acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     3121 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3181 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3241 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     3301 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3361 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3421 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3481 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3541 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3601 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3661 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3721 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3781 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3841 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3901 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3961 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4021 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4081 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4141 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4201 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4261 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4321 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4381 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4441 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4501 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4561 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4621 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4681 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4741 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4801 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4861 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4921 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4981 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5041 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5161 tttacggttc ctggcctttt gctggccttt tgctcacatg gctcgacaga tct
//
