LOCUS       sequence-115590-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mouse EPHB2 and GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Cortina C, Palomo-Ponce S, Iglesias M, Fernandez-Masip JL, Vivancos 
            A, Whissell G, Huma M, Peiro N, Gallego L, Jonkheer S, Davy A, 
            Lloreta J, Sancho E, Batlle E
  TITLE     EphB-ephrin-B interactions suppress colorectal cancer progression by
            compartmentalizing tumor cells.
  JOURNAL   Nat Genet. 2007 Nov;39(11):1376-83. Epub 2007 Sep 30.
  PUBMED    17906625
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2007-11"; volume: "39(11)"; pages: "1376-83. Epub 
            2007 Sep 30"
COMMENT     Sequence Label: FUW-ubiquitin-EphB2-SV40-GFP (B2G)
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(29..239)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      35..170
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(40..337)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        187..378
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     335..355
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cttgctcacc atggtggcta gctgcaaaag cctaggcctc caaaaaagcc tcctcactac
       61 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
      121 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
      181 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
      241 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
      301 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacagc
      361 tggttctttc cgcctcagaa ggtacctaac caagttcctc tttcagaggt tatttcaggc
      421 catggtgctg cgcaccggtt caaacctcta cagactggat ctggttcatc tgggcccgca
      481 tcacctggat actgttcagg atttttttct ggtggccagc tagagtgacc ccaacgcgga
      541 gaatgtcctc catcatcatc tgagatacaa cgtcgaaaga ggtgaagccg gcgttggcaa
      601 agctctcctt gtactggccc atcttgatgg cctctagcca ctcatccact gtgttaaagc
      661 tggtgtagtc cggtatcgtg cggtccagca gtggcaggtt gatgccagag gacaggggtg
      721 ccatggcttt gaggctgttg gggtttcgga tcatcttgtc cagcgtgttg acaatctggc
      781 cgaacttggg ccggtggttg cggtccttct gccagcagtc cagcatgagc tggtgcaggg
      841 cgctagggca gtccatgggc ggaggtagtc tgtagtcctg ttcaatggcg ttgattacgt
      901 cttgattggt catgtcc
//
