LOCUS       sequence-115594-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 10-12 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX4-25
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(396..413)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(396..412)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(405..427)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      476..562
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      654..681
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggaggtgcgt cacccggcaa ccttgggtag cagcgaagtc gaggcatttc tgtcctggct
       61 ggtctagacg tctcggccat ggtcctggca cagcaccggc aacagccgct gcaccgtttc
      121 gagcgcttgc ttgccgccaa tgttgctggc gatagccacc acttggtccg gggtcaggcc
      181 atggtcctgg cacagcaccg gcaacagccg ctgcaccgtt tcgagcgctt gcttgccgcc
      241 attgttgctg gcgatagcca ccacttggtc cggggtcagg ccatggtcct ggcacagcac
      301 cggcaacagc cgctgcaccg tttcgagcgc ttgcttgccg ccatcgtggc tggcgatagc
      361 caccacttgg tccggggtca ggggagacgc ttaagactgg ccgtcgtttt acaacgtcgt
      421 gactgggaaa acatccatgc tagcgttaac gcgagagtag ggaactgcca ggcatcaaat
      481 aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      541 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgtga agcaacggcc
      601 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaacta agcagaaggc
      661 catcctgacg gatggccttt ttgcgtttct acaaactctt cctggcta
//
