LOCUS       sequence-115595-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses calmodulin binding protein-LovTAP N538E fusion protein in 
            E. coli cells. LovTAP N358E is a optogenetic protein that can 
            repress the Trp promoter in the presence of blue light..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Strickland D, Yao X, Gawlak G, Rosen MK, Gardner KH, Sosnick TR
  TITLE     Rationally improving LOV domain-based photoswitches.
  JOURNAL   Nat Methods. 2010 Aug;7(8):623-6. doi: 10.1038/nmeth.1473. Epub 2010
            Jun 20.
  PUBMED    20562867
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1473"; journalName: "Nat Methods"; date: "2010-08"; 
            volume: "7"; issue: "8"; pages: "623-6"
COMMENT     Sequence Label: LovTAP N538E
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..220
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             221..238
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgaagcgacg atggaaaaag aatttcatag ccgtctcagc
      181 agccaaccgc tttaagaaaa tctcatcctc cggggcactt ctggttccgc gtggatcctt
      241 ggctactaca cttgaacgta ttgagaagaa ctttgtcatt actgacccaa gattgccaga
      301 taatcccatt atattcgcgt ccgatagttt cttgcagttg acagaatata gccgtgaaga
      361 aattttggga agaaactgca ggtttctaca aggtcctgaa actgatcgcg cgacagtgag
      421 aaaaattaga gatgccatag ataaccaaac agaggtcact gttcagctga ttaattatac
      481 aaagagtggt aaaaagttct ggaacctctt tcacttgcag cctatgcgag atcagaaggg
      541 agatgtccag tactttattg gggttcagtt ggatggaact gagcatgtcc gagatgctgc
      601 cgagagagag ggagtcatgc tgattaagaa aactgcagaa gagattgatg aggcggcatt
      661 tgtcgacctg cttaagaatg cctaccaaaa cgatctccat ttaccgttgt taaacctgat
      721 gctgacgcca gatgagcgcg aagcgttggg gactcgcgtg cgtattgtcg aagagctgtt
      781 gcgcggcgaa atgagccagc gtgagttaaa aaatgaactc ggcgc
//
