LOCUS       sequence-115607-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses calmodulin binding protein-LovTAP N538E fusion protein in 
            E. coli cells. LovTAP N358E is a optogenetic protein that can 
            repress the Trp promoter in the presence of blue light..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Strickland D, Yao X, Gawlak G, Rosen MK, Gardner KH, Sosnick TR
  TITLE     Rationally improving LOV domain-based photoswitches.
  JOURNAL   Nat Methods. 2010 Aug;7(8):623-6. doi: 10.1038/nmeth.1473. Epub 2010
            Jun 20.
  PUBMED    20562867
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1473"; journalName: "Nat Methods"; date: "2010-08"; 
            volume: "7"; issue: "8"; pages: "623-6"
COMMENT     Sequence Label: LovTAP N538E
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(713..730)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(731..808)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     RBS             819..841
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    856..880
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(880..899)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(881..899)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgggctttgt tagcagccgg atctaagctt aatcgctttt cagcaacacc tcttccagcc
       61 actggcgcag ctcgacgggc gcggctttca ggctgttaga tccacgcgta atcgtcgcga
      121 tgcctgcgcc gagttcattt tttaactcac gctggctcat ttcgccgcgc aacagctctt
      181 cgacaatacg cacgcgagtc cccaacgctt cgcgctcatc tggcgtcagc atcaggttta
      241 acaacggtaa atggagatcg ttttggtagg cattcttaag caggtcgaca aatgccgcct
      301 catcaatctc ttctgcagtt ttcttaatca gcatgactcc ctctctctcg gcagcatctc
      361 ggacatgctc agttccatcc aactgaaccc caataaagta ctggacatct cccttctgat
      421 ctcgcatagg ctgcaagtga aagaggttcc agaacttttt accactcttt gtataattaa
      481 tcagctgaac agtgacctct gtttggttat ctatggcatc tctaattttt ctcactgtcg
      541 cgcgatcagt ttcaggacct tgtagaaacc tgcagtttct tcccaaaatt tcttcacggc
      601 tatattctgt caactgcaag aaactatcgg acgcgaatat aatgggatta tctggcaatc
      661 ttgggtcagt aatgacaaag ttcttctcaa tacgttcaag tgtagtagcc aaggatccac
      721 gcggaaccag aagtgccccg gaggatgaga ttttcttaaa gcggttggct gctgagacgg
      781 ctatgaaatt ctttttccat cgtcgcttca tatgtatatc tccttcttaa agttaaacaa
      841 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
      901 tttcgcggga tcgagatctc gatcctctac gccgg
//
