LOCUS       sequence-115619-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of GATA6 Znc-finger mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Whissell G, Montagni E, Martinelli P, Hernando-Momblona X, Sevillano
            M, Jung P, Cortina C, Calon A, Abuli A, Castells A, Castellvi-Bel S,
            Nacht AS, Sancho E, Stephan-Otto Attolini C, Vicent GP, Real FX, 
            Batlle E
  TITLE     The transcription factor GATA6 enables self-renewal of colon adenoma
            stem cells by repressing BMP gene expression.
  JOURNAL   Nat Cell Biol. 2014 Jul;16(7):695-707. doi: 10.1038/ncb2992. Epub 
            2014 Jun 22.
  PUBMED    24952462
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2992"; 
            journalName: "Nat Cell Biol"; date: "2014-07"; volume: "16"; issue: 
            "7"; pages: "695-707"
COMMENT     Sequence Label: pLENTI-PGK-HYGRO-GATA6mut-HA
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    52..76
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(81..107)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgagcgg ccgccactgt gctggatatc aaccactttg
       61 tacaagaaag ctgggtctta agcgtaatct ggaacatcgt atgggtaggc cagggccagg
      121 gcgcaccagg aatccggtcg cacggaggac gtgacttcgg ccggcgaggc gagactgacg
      181 cctatgtaga gcccatcttg acccgaatac ttgagctcgc tgttctcggg attggtgctc
      241 tctcccgcac cagtcatcac cggggcaccc gcccctgagg ctgtaggttg tgttgtgggg
      301 gaagtatttt tgctgcaatc atctgagtta gaagaggtgg aagttggagt catgggaatg
      361 gaattattgc tattaccaga gcaagtcttt gatttattta tgttcttagg ttttcgtttc
      421 ctggtttgaa ttccctcttt tttcatagca agtggtctgg gcaccccatg gagtttcatg
      481 tagagtcctg cagcatttgc cacgggttca ccctcggcgt ttctgcgcca taaggtggta
      541 gttgtggtgt gtgcgttggc tgcggacaat ccaagccgcc gtgatgaagg cacgcgcttc
      601 tgcggcttga tgaggggccg gctgaggccg ttcatcttgc tgtagagccc tgcggcgttt
      661 gccaggtagt ggccggtgcc gtcccgccgc cacagcggcg tctggatgga gcctgcgttc
      721 actgcctcgc ggctctcgga caggtcctcc agcaggtctg cactgggacc ccggggcacc
      781 gggagcgggg ctccggcgcg gctctgcagg ctgtgcagca ccgg
//
