LOCUS       sequence-115621-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 
            rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Smith RH, Levy JR, Kotin RM
  TITLE     A simplified baculovirus-AAV expression vector system coupled with 
            one-step affinity purification yields high-titer rAAV stocks from 
            insect cells.
  JOURNAL   Mol Ther. 2009 Nov;17(11):1888-96. doi: 10.1038/mt.2009.128. Epub 
            2009 Jun 16.
  PUBMED    19532142
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2009.128"; journalName: "Mol Ther"; date: "2009-11"; 
            volume: "17"; issue: "11"; pages: "1888-96"
COMMENT     Sequence Label: pSR660
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(228..239)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gcatgctatg catcagctgc tagccgcaga gaccaaagtt caactgaaac gaatcaaccg
       61 gtttattgat taacaggcaa ttacagatta cgggtgaggt aacgggtgcc aatggggcgg
      121 ggttcagagt acacgccttc tgtattaaca gcaaagtcca cacttgtaga tttgtagtag
      181 ttggaggtgt actggatctc ggggttccag cgcttgctgt tttccttctg cagctcccat
      241 tcaatttcca cgctgacctg tccggtgctg tattgcgtga tgaaagagtt cagctttgac
      301 tggttgaagg tggtcggagg atccgcaggt acaggcgtgt tcttgatcag gatctgaggc
      361 ggaggatgtt tcaggccaaa gccgcccatc agcggagacg ggtggaagtt gccgtccgtg
      421 tgaggaatct tggcccagat gggaccctgc aggtacacgt cccggttctg ccagaccata
      481 ccgggtaagg ccccctggct gttgacagtt ccaatttgag gagccgtgtt ttgctgctgc
      541 aagttatctg ccacgatacc gtattcctct gtagccacag ggttagtggt tttgatttct
      601 tcctcgctgg tgagcatgac atcgctgtaa tccgcattgt ctctggcagc attttgtttg
      661 ccaaaaatca ggatcccgtt actgggaaaa aaacgctcct cgtcgtcttt gtgtgttgcc
      721 atagcgatgc caggattagc caatgaattt cttccattca gatggtattt ggtcccagca
      781 gtccaggcaa agttgctatt gttgttttgc ccggttgtcg ttgagacgcg ttgttggcgg
      841 taacagggtc ctggcagcca gttctttgcc tgattggcca ttgtatt
//
