LOCUS       sequence-115622-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ecadherin reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: SBP-EGFP-Ecadherin_puromycin
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     135..194
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             195..305
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(360..381)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(621..640)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat cgatatctgc ggcctagcta
      121 gcgggaggcg cgccatgtac aggatgcaac tcctgtcttg cattgcacta agtcttgcac
      181 ttgtcacgaa ttccgacgag aagaccactg gttggcgagg tggacacgtt gttgaaggac
      241 tggctgggga acttgaacaa cttcgtgcac gactggagca tcacccacaa ggtcaacgtg
      301 aaccacctgc aggtatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      361 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      421 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      481 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      541 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      601 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      661 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      721 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcat
//
