LOCUS       sequence-115627-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 13-14 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusB2-1
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(318..335)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(318..334)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(327..349)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      398..484
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      576..603
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcgttcaccc ggcaaccttg ggtagcagcg aagtcgaggc atttctgtcc tggctggtct
       61 agacgtctcg atagccacca cttggtccgg ggtcaggcca tggtcctggc acagcaccgg
      121 caacagccgc tgcaccgttt cgagcgcttg cttgccgcca atgttgctgg cgatagccac
      181 cacttggtcc ggggtcaggc catggtcctg gcacagcacc ggcaacagcc gctgcaccgt
      241 ttcgagcgct tgcttgccgc caatgttgct ggcgatagcc accacttggt ccggggtcag
      301 gccatgagac gcttaagact ggccgtcgtt ttacaacgtc gtgactggga aaacatccat
      361 gctagcgtta acgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
      421 ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
      481 aaatccgccg ggagcggatt tgaacgttgt gaagcaacgg cccggagggt ggcgggcagg
      541 acgcccgcca taaactgcca ggcatcaaac taagcagaag gccatcctga cggatggcct
      601 ttttgcgttt ctacaaactc ttcctggcta gcggtacgcg tattaattgc gttgcgctca
      661 ctgcccgctt tccagtcggg aaa
//
