LOCUS       sequence-115628-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TNF reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: TNF-SBP-mCherry_puromycin
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat cgatatctgc ggcctagcta
      121 gcgggaggcg cgccatgtct accgagtcta tgattaggga cgtggaactg gctgaggagg
      181 cactgcccaa aaaaaccggc ggaccacagg gctctaggag atgtctgttt ctgtctctgt
      241 tctcttttct catcgtggct ggcgctacaa cactcttctg tctgctccat ttcggcgtga
      301 ttggaccaca gcgagaggaa tttccccggg atctgtcact catttcacca ctggcacagg
      361 ctgtccgatc ttcatctcgg actccatccg acaaacctgt cgcccatgtc gtcgccaacc
      421 cacaggccga gggccagctc cagtggctca ataggagggc aaacgctctg ctcgccaatg
      481 gcgtggaact ccgggataac cagctcgtcg tgcctagtga gggactgtac ctcatctact
      541 cccaggtgct gtttaagggc cagggatgtc cttctacaca tgtgctgctc acacacacaa
      601 tttcacggat cgccgtgtct taccagacta aagtcaatct gctctctgcc atcaaatccc
      661 catgtcagcg ggaaacacct gagggcgctg aggctaaacc ttggtacgaa cccatctacc
      721 tcggaggcgt gttccagctg gagaagggcg atagactgag tgctgagatc aatcgacccg
      781 actacctcga c
//
