LOCUS       sequence-115630-         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 13-14 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusB2-9
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(317..334)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(317..333)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(326..348)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      397..483
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      575..602
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcgtcacccg gcaaccttgg gtagcagcga agtcgaggca tttctgtcct ggctggtcta
       61 gacgtctcga tagccaccac ttggtccggg gtcaggccat ggtcctggca cagcaccggc
      121 aacagccgct gcaccgtttc gagcgcttgc ttgccgccaa tgttgctggc gatagccacc
      181 acttggtccg gggtcaggcc atggtcctgg cacagcaccg gcaacagccg ctgcaccgtt
      241 tcgagcgctt gcttgccgcc attgttgctg gcgatagcca ccacttggtc cggggtcagg
      301 ccatgagacg cttaagactg gccgtcgttt tacaacgtcg tgactgggaa aacatccatg
      361 ctagcgttaa cgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
      421 gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
      481 aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggagggtg gcgggcagga
      541 cgcccgccat aaactgccag gcatcaaact aagcagaagg ccatcctgac ggatggcctt
      601 tttgcgtttc tacaaactct tcctggctag cggtacgcgt attaattgcg tt
//
