LOCUS       sequence-115631-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TNF reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: TNF-SBP-mCherry_puromycin
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(114..227)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatacctgc aggtggttca
      121 cgttgacctt gtgggtgatg ctccagtcgt gcacgaagtt gttcaagttc cccagccagt
      181 ccttcaacaa cgtgtccacc tcgccaacca gtggtcttct cgtccatgga attcaaaggc
      241 ctaggtgcgc catgagctcg gccctcgagg cccagagcaa tgatgccaaa gtagacctgg
      301 ccagattcgg caaagtcgag gtagtcgggt cgattgatct cagcactcag tctatcgccc
      361 ttctccagct ggaacacgcc tccgaggtag atgggttcgt accaaggttt agcctcagcg
      421 ccctcaggtg tttcccgctg acatggggat ttgatggcag agagcagatt gactttagtc
      481 tggtaagaca cggcgatccg tgaaattgtg tgtgtgagca gcacatgtgt agaaggacat
      541 ccctggccct taaacagcac ctgggagtag atgaggtaca gtccctcact aggcacgacg
      601 agctggttat cccggagttc cacgccattg gcgagcagag cgtttgccct cctattgagc
      661 cactggagct ggccctcggc ctgtgggttg gcgacgacat gggcgacagg tttgtcggat
      721 ggagtccgag atgaagatcg gacagcctgt gccagtggtg aaatgagtga cagat
//
