LOCUS       sequence-115639-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 10-12 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX4-10
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(392..409)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(392..408)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(401..423)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      472..558
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      650..677
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgcgtcacc cggcaacctt gggtagcagc gaagtcgagg catttctgtc ctggctggtc
       61 tagacgtctc ggccatggtc ctggcacagc accggcaaca gccgctgcac cgtttcgagc
      121 gcttgcttgc cgccatcgtg gctggcgata gccaccactt ggtccggagt caggccatgg
      181 tcctggcaca gcaccggcaa cagccgctgc accgtttcga gcgcttgctt gccgccattg
      241 ttgctggcga tagccaccac ttggtccggg gtcaggccat ggtcctggca cagcaccggc
      301 aacagccgct gcaccgtttc gagcgcttgc ttgccgccaa tgttgctggc gatagccacc
      361 acttggtccg gggtcagggg agacgcttaa gactggccgt cgttttacaa cgtcgtgact
      421 gggaaaacat ccatgctagc gttaacgcga gagtagggaa ctgccaggca tcaaataaaa
      481 cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      541 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga
      601 gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc
      661 ctgacggatg gcctttttgc gtttctacaa actcttcctg gctagcggta cgcgtattaa
      721 ttg
//
