LOCUS       sequence-115643-        5959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of mouse Oct3/4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Aug 25. 126(4):663-76."
COMMENT     Sequence Label: pMXs-Oct3/4
FEATURES             Location/Qualifiers
     source          1..5959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     protein_bind    906..930
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2159..2183)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2234..2608
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(2280..2296)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(2618..3034)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2634..2650)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2666..2688)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3093..3450
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             3514..4030
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     promoter        4061..4165
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4166..4234,4235..5026)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4384..4403)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5190..5778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5679..5698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5932..5949
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgtcgacc actgtgctgg cggccgctcg
      781 agtttaaata gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg
      841 gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcagctgga
      901 atatcaccac tttgtacaag aaagctgggt tgatcaacag catcactgag cttctttccc
      961 catcccaccc cccacccctg ttgtgctttt aatccctcct cagtaaaaga atttaacccc
     1021 aaagctccag gttctcttgt ctacctccct tgccttggct cacagcatcc ccagggaggg
     1081 ctggtgcctc agtttgaatg catgggagag cccagagcag tgacgggaac agagggaaag
     1141 gcctcgccct caggaaaagg gactgagtag agtgtggtga agtgggggct tccatagcct
     1201 ggggtgccaa agtggggacc tgggggcaga ggaaaggata cagccccccc tgggaaaggt
     1261 gtccctgtag cctcatactc ttctcgttgg gaatactcaa tacttgatct tttgcccttc
     1321 tggcgccggt tacagaacca tactcgaacc acatccttct ctagcccaag ctgattggcg
     1381 atgtgagtga tctgctgtag ggagggcttc gggcacttca gaaacatggt ctccagactc
     1441 cacctcacac ggttctcaat gctagttcgc tttctcttcc gggcctgcac cagggtctcc
     1501 gatttgcata tctcctgaag gttctcattg ttgtcggctt cctccaccca cttctccagc
     1561 aggggccgca gcttacacat gttcttaagg ctgagctgca aggcctcgaa gcgacagatg
     1621 gtggtctggc tgaacacctt tccaaagaga acgcccaggg tgagccccac gtcggcctgg
     1681 gtgtacccca aggtgatcct cttctgcttc agcagcttgg caaactgttc tagctccttc
     1741 tgcagggctt tcatgtcctg ggactcctcg ggagttggtt ccaccttctc caacttcacg
     1801 gcattggggc ggtcggcaca gggctcagag gaggttccct ctgagttgct ttccactcgt
     1861 gctcctgcct ggccctcagg ctgcaaagtc tccacgccaa cttgggggac taggcccagt
     1921 ccaacctgag gtccacagta tgccatccct ccgcagaact cgtatgcggg cggacatggg
     1981 gagatcccca atacctctga gcctggtccg attccaggcc cacctggagg cccttggaag
     2041 cttagccagg ttcgagaatc cacccagccc ggctccagcc ctgctgaccc atcaccccca
     2101 cctggtgggg gtgagaaggc gaagtctgaa gccaggtgtc cagccatggg gaaggtggag
     2161 cctgcttttt tgtacaaact tgtgatttcc cgtaccacca cactgggatc cttaattaac
     2221 tagctagatc cggcagtcta gaggatggtc cacccccggg gtcggcagcc ttcacgtggg
     2281 cggcgtgtat ccaagctgcg atgccgtcta ctttgagggc ggtgggggtg gtcagcagga
     2341 ctgtgtaagg tcctttccag cgaggttcta ggttcttagt ctggtgtcgg cggacccaca
     2401 ctgtgtcgcc gactcggtaa gggtgaggta ccaccggtcg gtccagttgt tcttggtagg
     2461 ctgccgccag aggtctccag acttcgtgct ggactaagta gagagcctgt aagtgagctt
     2521 ggagagaggg gctgttagta actcttgtca tgtcagggtc agggaagttt acaaggggcg
     2581 ggggtgcccc atataagatc tcatatggcc atatgggggc gcctagagaa ggagtgaggg
     2641 ctggataaag ggaggatcga ggcggggtcg aacgaggagg ttcaaggggg agagacgggg
     2701 cggatggagg aagaggaggc ggaggcttag ggtgtacaaa gggcttgacc cagggagggg
     2761 ggtcaaaagc caaggcttcc caggtcacga tgtaggggac ctggtctggg tgtccatgcg
     2821 ggccaggtga aaagaccttg atcttaacct gggtgatgag gtctcggtta aaggtgccgt
     2881 ctcgcggcca tccgacgtta aaggttggcc attctgcaga gcagaaggta acccaacgtc
     2941 tcttcttgac atctaccgac tggttgtgag cgatccgctc gacatctttc cagtgaccta
     3001 aggtcaaact taagggagtg gtaacagtct ggccctaatt ttcagacaaa tacagaaaca
     3061 cagtcagaca gagacaacac agaacgatgc tgcagcagac aagacgcgcg gcgcggcttc
     3121 ggtcccaaac cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg ttctcgtctc
     3181 ctaccagaac cacatatccc tcctattagg ggggtgcacc aaagagtcca aaacgatcgg
     3241 gatttttgga ctcaggtcgg gccacaaaaa cggcccccga agtccctggg acgtctccca
     3301 gggttgcggc cgggtgttcc gaactcgtca gttccaccac gggtccgcca gatacagagc
     3361 tagttagcta actagtaccg acgcaggcgc ataaaatcag tcatagacac tagacaatcg
     3421 gacagacaca gataagttgc tggccagctt acctcccggt ggtgggtcgg tggtccctgg
     3481 gcaggggtct cccgatcccg gacgagcccc caaatgaaag acccccgctg acgggtagtc
     3541 aatcactcag aggagaccct cccaaggaac agcgagacca caagtcggat gcaactgcaa
     3601 gagggtttat tggatacacg ggtacccggg cgactcagtc aatcggagga ctggcgcccc
     3661 gagtgagggg ttgtgggctc ttttattgag ctcggggagc agaagcgcgc gaacagaagc
     3721 gagaagcgaa ctgattggtt agttcaaata aggcacaggg tcatttcagg tccttggggc
     3781 accctggaaa catctgatgg ttctctagaa actgctgagg gctggaccgc atctggggac
     3841 catctgttct tggccctgag ccggggcagg aactgcttac cacagatatc ctgtttggcc
     3901 catattcagc tgttccatct gttcctgacc ttgatctgaa cttttctatt ctcagttatg
     3961 tatttttcca tgccttgcaa aatggcgtta cttaagctag cttgccaaac ctacaggtgg
     4021 ggtctttcat tatgcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4081 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4141 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4201 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4261 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4321 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4381 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4441 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4501 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4561 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4621 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4681 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4741 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4801 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4861 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4921 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4981 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5041 ttactcatat atactttaga ttgatttgcg gccggccgca aaaggatcta ggtgaagatc
     5101 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5161 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5221 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5281 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5341 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5401 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5461 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5521 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5581 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5641 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5701 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5761 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5821 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5881 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5941 gtgagcgagg aagcggaag
//
