LOCUS       sequence-115647-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synchronize trafficking of ST from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Str-KDEL_ST-SBP-EGFP
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             290..403
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(458..479)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(719..738)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
       61 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
      121 gataagcttg ccacaacccg ggaggcgcgc catgattcac accaacctga agaaaaagtt
      181 cagctgctgc gtcctggtct ttcttctgtt tgcagtcatc tgtgtgtgga aggaaaagaa
      241 gaaagggagt tactatgatt cctttaaatt gcaaaccaag gggaattcca tggacgagaa
      301 gaccactggt tggcgaggtg gacacgttgt tgaaggactg gctggggaac ttgaacaact
      361 tcgtgcacga ctggagcatc acccacaagg tcaacgtgaa ccacctgcag gtatggtgag
      421 caaaggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      481 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      541 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      601 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      661 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      721 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgaca
//
