LOCUS       sequence-115651-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for human KCC2 under CAG promoter. 
            Coexpression of TdTomato with IRES sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Bortone D, Polleux F
  TITLE     KCC2 expression promotes the termination of cortical interneuron 
            migration in a voltage-sensitive calcium-dependent manner.
  JOURNAL   Neuron. 2009 Apr 16;62(1):53-71. doi: 10.1016/j.neuron.2009.01.034.
  PUBMED    19376067
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.01"; journalName: "Neuron"; date: 
            "2009-04-16- 16"; volume: "62"; issue: "1"; pages: "53-71"
COMMENT     Sequence Label: pCITF-KCC2
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      160..169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(299..317)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     567..586
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     761..784
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca
       61 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
      121 ctttgaaaaa cacgatgata atatggatcg ctagctgccg ccaccatggt gagcaagggc
      181 gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
      241 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      301 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
      361 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
      421 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg
      481 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg
      541 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      601 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc
      661 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag
      721 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac
      781 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc
      841 ctggggcatg gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccga
//
