LOCUS       sequence-11566-e        8760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte 
            EM, Voigt CA, Ellington AD
  TITLE     A synthetic genetic edge detection program.
  JOURNAL   Cell. 2009 Jun 26. 137(7):1272-81.
  PUBMED    19563759
REFERENCE   2  (bases 1 to 8760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 26. 137(7):1272-81."
COMMENT     Sequence Label: pJT104
FEATURES             Location/Qualifiers
     source          1..8760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             106..117
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             138..3182
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGSLSQMWIGDKNQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(140..162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3217..3288
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3304..3331
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             3479..4189
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     CDS             4190..4222
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      4295..4366
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4382..4409
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    4424..4442
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4449..4467
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             4486..4497
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5301..5372
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5388..5415
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    5422..5442
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(5526..5543)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(5805..6755)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      6803..7025
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(7517..7611)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(7641..8432,8433..8501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8264..8283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8502..8606)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8671..8689
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8693..8736)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    8739..8760
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagagatgat agatcccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      181 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      241 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt
      301 ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata
      361 ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg
      421 tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt
      481 actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt
      541 ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc
      601 aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc
      661 gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt
      721 ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca
      781 gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta ctggaggctg
      841 aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg
      901 gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg
      961 gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg
     1021 ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga
     1081 ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc
     1141 tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc
     1201 atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca
     1261 actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg
     1321 accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa
     1381 tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa
     1441 tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag
     1501 gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc
     1561 cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt
     1621 acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat
     1681 ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg
     1741 gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac
     1801 agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca
     1861 acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta
     1921 tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc
     1981 agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc
     2041 tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc
     2101 tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc
     2161 ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac
     2221 cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg
     2281 cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca
     2341 gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag
     2401 gctctctttc acagatgtgg attggcgata aaaaccaact gctgacgccg ctgcgcgatc
     2461 agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc
     2521 ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt
     2581 tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct cacgcgtggc
     2641 agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc
     2701 aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg
     2761 gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc
     2821 aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt
     2881 cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg
     2941 aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca
     3001 gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat
     3061 ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag
     3121 tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa
     3181 aagcggccgc tcgagtctaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     3241 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     3301 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct tgcatttaca
     3361 ttttgaaaca tctatagcga taaatgaaac atcttaaaag ttttagtatc atattcgtgt
     3421 tggattattc tgcatttttg gggagaatgg acttactaga gtcacacagg acggccggat
     3481 gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc
     3541 aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat
     3601 ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta
     3661 taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat
     3721 cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga
     3781 gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac
     3841 ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc
     3901 attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt
     3961 tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg
     4021 catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca
     4081 ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc
     4141 cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga
     4201 cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat cgcctctgat
     4261 agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     4321 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     4381 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat cagtgataga
     4441 gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga ggagaaatac
     4501 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt
     4561 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa
     4621 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta
     4681 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat
     4741 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac
     4801 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc
     4861 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca
     4921 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca
     4981 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca
     5041 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc
     5101 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc
     5161 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta
     5221 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga
     5281 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     5341 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     5401 gtgggccttt ctgcgtttat atactagtag cggccgctgc agtccggcaa aaaaacgggc
     5461 aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag
     5521 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5581 cactcaaagg cggtaatctc gaggttacat tgtcgatctg ttcatggtga acagctttaa
     5641 atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg ttttcatctg
     5701 tgcatatgga cagttttccc tttgatatct aacggtgaac agttgttcta cttttgtttg
     5761 ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat ccttccgtat
     5821 ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca
     5881 ttgagatcat gcttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga
     5941 atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct
     6001 gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg
     6061 gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg
     6121 cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt
     6181 ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg
     6241 aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt
     6301 tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt
     6361 tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta
     6421 aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca
     6481 aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct
     6541 ttagctaata caccataagc attttcccta ctgatgttca tcatctgagc gtattggtta
     6601 taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt
     6661 gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct
     6721 agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt
     6781 taatcactat accaattgag atgggctagt caatgataat tacatgtcct tttcctttga
     6841 gttgtgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga
     6901 ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca agtggttata
     6961 atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat
     7021 aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct
     7081 cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc
     7141 aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct gtctttttcg
     7201 tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag
     7261 gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc ccgtcacggg
     7321 cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt ttgctgttca
     7381 gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt gacaggtcat
     7441 tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta cccgtcttac
     7501 tgtccctagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga
     7561 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     7621 tcgtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8581 aaaaataaac aaataggggt tccgcgcaca tttcccatgg tgccacctga cgtctaagaa
     8641 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
     8701 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag
//
