LOCUS       sequence-115661-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for human KCC2 under CAG promoter. 
            Coexpression of TdTomato with IRES sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Bortone D, Polleux F
  TITLE     KCC2 expression promotes the termination of cortical interneuron 
            migration in a voltage-sensitive calcium-dependent manner.
  JOURNAL   Neuron. 2009 Apr 16;62(1):53-71. doi: 10.1016/j.neuron.2009.01.034.
  PUBMED    19376067
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.01"; journalName: "Neuron"; date: 
            "2009-04-16- 16"; volume: "62"; issue: "1"; pages: "53-71"
COMMENT     Sequence Label: pCITF-KCC2
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(174..197)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(281..304)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(479..498)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     748..766
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatcc tctagagtcg cggccaattc gcccttgacg cgtgcggccg ctacttgtca
      181 tcatcgtcct tatagtcctt gtacagctcg tccatgccgt acaggaacag gtggtggcgg
      241 ccctcggagc gctcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc
      301 agcttggtgt ccacgtagta gtagccgggc agttgcacgg gcttcttggc catgtagatg
      361 gtcttgaact ccaccaggta gcggccgccg tccttcagct tcagggcctg gtggatctcg
      421 cccttcagca cgccgtcgcg ggggtacagg cgctcggtgg aggcctccca gcccatggtc
      481 ttcttctgca ttacggggcc gtcggggggg aagttggtgc cgcgcatctt caccttgtag
      541 atcagcgtgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccagacc gccgtcctcg
      601 aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttctt gtaatcgggg
      661 atgtcggcgg ggtgcttcac gtacgccttg gagccgtaca tgaactgggg ggacaggatg
      721 tcccaggcga agggcagggg gccgcccttg gtcaccttca gcttggcggt ctgggtgccc
      781 tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt catggagccc
      841 tccatgcgca ccttgaagcg catgaactct ttgatgacgg ccatgttgtt gtcctcggag
      901 gaggcggtgc cgga
//
