LOCUS       sequence-115667-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 13-14 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusB2-2
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(322..339)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(322..338)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(331..353)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      402..488
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      580..607
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagacgt ctcgatagcc accacttggt ccggggtcag gccatggtcc tggcacagca
      121 ccggcaacag ccgctgcacc gtttcgagcg cttgcttgcc gccatcgtgg ctggcgatag
      181 ccaccacttg gtccggagtc aggccatggt cctggcacag caccggcaac agccgctgca
      241 ccgtttcgag cgcttgcttg ccgccaatgt tgctggcgat agccaccact tggtccgggg
      301 tcaggccatg agacgcttaa gactggccgt cgttttacaa cgtcgtgact gggaaaacat
      361 ccatgctagc gttaacgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
      421 agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
      481 ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga gggtggcggg
      541 caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc ctgacggatg
      601 gcctttttgc gtttctacaa actcttcctg gctagcggta cgcgtattaa ttgcgttgcg
      661 ctcactgccc gctttccagt cgggaaa
//
