LOCUS       sequence-115668-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 13-14 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusB2-10
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(319..336)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(319..335)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(328..350)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      399..485
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      577..604
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgcgtcacc cggcaacctt gggtagcagc gaagtcgagg catttctgtc ctggctggtc
       61 tagacgtctc gatagccacc acttggtccg gggtcaggcc atggtcctgg cacagcaccg
      121 gcaacagccg ctgcaccgtt tcgagcgctt gcttgccgcc atcgtggctg gcgatagcca
      181 ccacttggtc cggagtcagg ccatggtcct ggcacagcac cggcaacagc cgctgcaccg
      241 tttcgagcgc ttgcttgccg ccattgttgc tggcgatagc caccacttgg tccggggtca
      301 ggccatgaga cgcttaagac tggccgtcgt tttacaacgt cgtgactggg aaaacatcca
      361 tgctagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
      421 cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
      481 caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag
      541 gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg acggatggcc
      601 tttttgcgtt tctacaaact cttcctggct a
//
