LOCUS       sequence-115669-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse Oct4 targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ochiai H, Sugawara T, Yamamoto T
  TITLE     Simultaneous live imaging of the transcription and nuclear position 
            of specific genes.
  JOURNAL   Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
  PUBMED    26092696
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 19. pii: gkv624."
COMMENT     Sequence Label: pTV-Oct4-TK-HMS
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(59..149)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(188..643)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(325..346)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     537..556
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     770..792
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     784..801
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     785..801
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     811..830
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        811..829
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
       61 tcttcctttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
      121 atttgaatgt atttagaaaa ataaacaaat agggattccg cgcacatttc cccgaaaagt
      181 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgctaaatc
      241 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
      301 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
      361 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
      421 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
      481 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
      541 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
      601 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
      661 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
      721 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
      781 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgtga
      841 tctcacggct cttaatgtag gcgcaagggg gtggggcatc ttaggagctg cttctccaca
      901 ggtaagggag gattagacgc ttgtagcttg aactgtcaga ggtgggggct tgggctccct
      961 tcttgctgcc tcactcactc tgtttgatcg gcctttcagg aaaggtgttc agccagacca
     1021 ccatctgtcg cttcgaggcc ttgcagctca gccttaaga
//
