LOCUS       sequence-11567-e        8627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte 
            EM, Voigt CA, Ellington AD
  TITLE     A synthetic genetic edge detection program.
  JOURNAL   Cell. 2009 Jun 26. 137(7):1272-81.
  PUBMED    19563759
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 26. 137(7):1272-81."
COMMENT     Sequence Label: pJT105
FEATURES             Location/Qualifiers
     source          1..8627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             106..117
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             138..3182
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGSLSQMWIGDKNQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(140..162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3217..3288
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3304..3331
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             3487..3498
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4084..4116
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      4164..4235
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4251..4278
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    4293..4311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4318..4336
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             4355..4366
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5170..5241
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5257..5284
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    5291..5311
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      5312..5369
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(5393..5410)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(5672..6622)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      6670..6892
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(7384..7478)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(7508..8299,8300..8368))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8131..8150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8369..8473)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8538..8556
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8560..8603)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    8606..8627
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tggagaaaga ggagaaatac
      121 tagagatgat agatcccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      181 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      241 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt
      301 ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata
      361 ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg
      421 tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt
      481 actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt
      541 ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc
      601 aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc
      661 gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt
      721 ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca
      781 gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta ctggaggctg
      841 aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg
      901 gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg
      961 gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg
     1021 ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga
     1081 ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc
     1141 tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc
     1201 atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca
     1261 actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg
     1321 accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa
     1381 tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa
     1441 tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag
     1501 gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc
     1561 cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt
     1621 acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat
     1681 ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg
     1741 gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac
     1801 agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca
     1861 acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta
     1921 tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc
     1981 agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc
     2041 tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc
     2101 tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc
     2161 ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac
     2221 cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg
     2281 cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca
     2341 gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag
     2401 gctctctttc acagatgtgg attggcgata aaaaccaact gctgacgccg ctgcgcgatc
     2461 agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc
     2521 ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt
     2581 tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct cacgcgtggc
     2641 agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc
     2701 aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg
     2761 gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc
     2821 aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt
     2881 cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg
     2941 aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca
     3001 gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat
     3061 ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag
     3121 tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa
     3181 aagcggccgc tcgagtctaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     3241 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     3301 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct tgcatttaca
     3361 ttttgaaaca tctatagcga taaatgaaac atcttaaaag ttttagtatc atattcgtgt
     3421 tggattattc tgcatttttg gggagaatgg actcgctgat agtgctagtg tagatcgcta
     3481 ctagagaaag aggagaaata ctagatgact ataatgataa aaaaatcgga ttttttggca
     3541 attccatcgg aggagtataa aggtattcta agtcttcgtt atcaagtgtt taagcaaaga
     3601 cttgagtggg acttagttgt agaaaataac cttgaatcag atgagtatga taactcaaat
     3661 gcagaatata tttatgcttg tgatgatact gaaaatgtaa gtggatgctg gcgtttatta
     3721 cctacaacag gtgattatat gctgaaaagt gtttttcctg aattgcttgg tcaacagagt
     3781 gctcccaaag atcctaatat agtcgaatta agtcgttttg ctgtaggtaa aaatagctca
     3841 aagataaata actctgctag tgaaattaca atgaaactat ttgaagctat atataaacac
     3901 gctgttagtc aaggtattac agaatatgta acagtaacat caacagcaat agagcgattt
     3961 ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca aagaaattca tgtattaggt
     4021 gacactaaat cggttgtatt gtctatgcct attaatgaac agtttaaaaa agcagtctta
     4081 aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt
     4141 agatctctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     4201 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     4261 cgggtgggcc tttctgcgtt tatatactag agtccctatc agtgatagag attgacatcc
     4321 ctatcagtga tagagatact gagcactact agagaaagag gagaaatact agatgaaaaa
     4381 cataaatgcc gacgacacat acagaataat taataaaatt aaagcttgta gaagcaataa
     4441 tgatattaat caatgcttat ctgatatgac taaaatggta cattgtgaat attatttact
     4501 cgcgatcatt tatcctcatt ctatggttaa atctgatatt tcaatcctag ataattaccc
     4561 taaaaaatgg aggcaatatt atgatgacgc taatttaata aaatatgatc ctatagtaga
     4621 ttattctaac tccaatcatt caccaattaa ttggaatata tttgaaaaca atgctgtaaa
     4681 taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca ctgggtttag
     4741 tttccctatt catacggcta acaatggctt cggaatgctt agttttgcac attcagaaaa
     4801 agacaactat atagatagtt tatttttaca tgcgtgtatg aacataccat taattgttcc
     4861 ttctctagtt gataattatc gaaaaataaa tatagcaaat aataaatcaa acaacgattt
     4921 aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat
     4981 ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc catttaacca atgcgcaaat
     5041 gaaactcaat acaacaaacc gctgccaaag tatttctaaa gcaattttaa caggagcaat
     5101 tgattgccca tactttaaaa attaataaca ctgatagtgc tagtgtagat cactactaga
     5161 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     5221 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     5281 tgcgtttata tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac
     5341 cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca
     5401 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5461 taatctcgag gttacattgt cgatctgttc atggtgaaca gctttaaatg caccaaaaac
     5521 tcgtaaaagc tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atatggacag
     5581 ttttcccttt gatatctaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc
     5641 ttcactgata gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt
     5701 tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatgct
     5761 tactttgcat gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta
     5821 aagcatcgtg tagtgttttt cttagtccgt tacgtaggta ggaatctgat gtaatggttg
     5881 ttggtatttt gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca
     5941 tttgtctatc tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa
     6001 ccaccaattt catattgctg taagtgttta aatctttact tattggtttc aaaacccatt
     6061 ggttaagcct tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat
     6121 caaggctaat ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc
     6181 actcataaat cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt
     6241 ttatgaattt ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta
     6301 atttttcgct tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca
     6361 aaggattcct gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac
     6421 cataagcatt ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata
     6481 ccgtccgttc tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata
     6541 aaattagctt ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt
     6601 tgaaaacaac taattcagac atacatctca attggtctag gtgattttaa tcactatacc
     6661 aattgagatg ggctagtcaa tgataattac atgtcctttt cctttgagtt gtgggtatct
     6721 gtaaattctg ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt
     6781 ccgctagacc tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa
     6841 agaaagaata aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt
     6901 agtcagttcc gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca
     6961 gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata
     7021 ttccttttgt ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc
     7081 gctgcgctca cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg
     7141 attcatgcaa ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt
     7201 tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc
     7261 tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa
     7321 tgcacccagt aaggcagcgg tatcatcaac aggcttaccc gtcttactgt ccctagtgct
     7381 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     7441 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg taaacttggt
     7501 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7561 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7621 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7681 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7741 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7801 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7861 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7921 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7981 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8041 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8101 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8161 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8221 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8281 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8341 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8401 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8461 taggggttcc gcgcacattt cccatggtgc cacctgacgt ctaagaaacc attattatca
     8521 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct
     8581 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagag
//
