LOCUS       sequence-115672-        3507 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression / in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Feike AC, Rachor K, Gentzel M, Schambony A
  TITLE     Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA.
  JOURNAL   Biochem Biophys Res Commun. 2010 Oct 1;400(4):500-6. doi: 
            10.1016/j.bbrc.2010.08.074. Epub 2010 Aug 21.
  PUBMED    20732301
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2010.08.074"; journalName: "Biochem Biophys Res 
            Commun"; date: "2010-10-1- 1"; volume: "400"; issue: "4"; pages: 
            "500-6"
COMMENT     Sequence Label: pCS2 xRor2-mCherry
FEATURES             Location/Qualifiers
     source          1..3507
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2797..3507
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2942..2960)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3210..3229
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgtccagga ccaggagcca gaatgggggg attggatgtg ccctgctcgg gcttctcgtg
       61 gctgccatac tgctgcctgt acaagcttca ggtgaaatgg aaataccaga cttgaatgac
      121 ccactgggac aaatggaaag ccacgacagg ctcggagcaa ctcctagagg atactatttg
      181 acgtttctag aacctgtgaa caatatcaca attgtgcaag gtcaggcagc aacactccat
      241 tgcaaagtgt ccgggaatcc actccccaat gtaaagtggc tgaagaacga tgcacctgtg
      301 gttcaggagc ccaagagaat taccattagg aaaacagact acggctcacg attacggata
      361 caagacctgg acaccaccga cactggctat tatcagtgtg tcgccaccaa tgggataaag
      421 accattactg caactggagt cctctttgtt aggcttggac caacaaacag ccctaacccg
      481 aacatccagg atgactacca caaagatggg ttttgtcagc cctacagggg aattgcctgc
      541 gcaagattca ttggcaacag gaccatatat gtcgactcac ttcagatgca aggggaaatc
      601 gaaaaccgaa ttacagctgc ctttacaatg attggcactt ccacccatct ctctgaccag
      661 tgctctcagt ttgcaatccc atcattttgc cacttcgtgt tccctctctg tgatgatcaa
      721 acttccaagc caagagagct gtgcagggat gagtgtgaag tgctggaaaa tgacctgtgc
      781 aggcaggagt acaacatcgc ccgctccaat cccctcattc tcatgcagct tcacctacca
      841 aactgcgaag aactgccatt gcctgaaagt cctgaagctg ccaactgcat gaggattggg
      901 atcccagtgg agaagcttaa cagatatcag cagtgttaca atgggactgg aactgattac
      961 agaggatctg tcagcgtcac taagtctggc catcagtgcc agccatggag tcatcaggtt
     1021 ccccatagtc attcactatc caatgcggat taccctgaaa ttggtggagg ccactcttac
     1081 tgcagaaatc caggaggcca gatggagggt ccttggtgtt tcacacagaa taaaaatgtc
     1141 cgcatggagc tatgcgatat tcctgcttgc cgtacacgtg acaacacaaa gatggagatt
     1201 ctctacatct tggtccccag cattgcaatc ccactggtca tcgcctgctt ctttttctta
     1261 gtatgcatgt gccgaaataa acagaaagca gaaggttcta caccgcagcg ccgccaactc
     1321 atggcctctc caagccagga catggaaatg ccccttatga atcagcacaa acagcagcca
     1381 aaagtaaaag agataaacct atcaactgta aggttcatgg aagagctagg tgaagatcgt
     1441 tttggaaaag tttacaaggg acatctcttt ggtaccactc caggggaaca gactcaaact
     1501 gttgccatca aaacacttaa agacaaagtt gaagttgctc ttcgtgaaga attcaaacat
     1561 gaggccatga tgcgatctag gcttcagcac ccaaatatag tttgtctgat tgggacagtg
     1621 acaaaggagc agcccatgag catgatattt agctattctc ctcttagtga tctgcacgag
     1681 ttcttggtca tgagatcacc acattcagat gtaggcagca cagatgacga taagactgtt
     1741 aaatccaccc tagagcccgc tgattttctt catattgtga cccagatagc atcaggaatg
     1801 gaatttcttt caagtcacca tgttgttcat aaagatttgg ctgccagaaa tgttttggtg
     1861 ttcgataaac tcagcattaa aatttcagat ctcggcctgt tccgggaagt atatgctgca
     1921 gattactata aactgatggg aaactccatg cttccgatta gatggatgtc tccagaagcg
     1981 atcacatatg gaaagtgttc tgttgattca gatatttggt cctatggggt cgtggtgtgg
     2041 gaaatcttca gctacgggct gcaaccgtat tgtgggtatt ccaaccagga tgtcattgaa
     2101 atgatccgaa atcgccaggt gctgctctgc cctgatgact gtcctgcatg gatatacact
     2161 ctaatgctgg agtgctggag tgaatttcct gctagaagac caagatttaa ggacatacac
     2221 acacggctaa gaacatggga aaatatgtcc aactataata gctctgcaca aacatctggg
     2281 gcaagtaata ctacccagac aagttctcta agcacaagcc cagtaagcaa tgttagcaat
     2341 gctaggtatg ttgggccaaa gccaaaaact cagccattcc aacagccaca gtttttgcaa
     2401 atgaaaggac aaattagacc tatggttcct caacctcaac tctacattcc agttaatggg
     2461 tatcaacaga tggctgctta tttttaccca gttcagatac caatgcaaat ggctccacaa
     2521 caaatgccac ctcagatcat acccaaacct ggatcccacc acagtggaag tggttccacc
     2581 agtacaggat atgtcacaac agcgccctca aataattcca tggctgacag ggttgctctc
     2641 cttgcagatg gggcagatga agcacagtta acggcagaag atatgtctcc aaatcctggt
     2701 caggaagaag aaggttctgt tccagaaaca gaattgctgg gtgacaatga cacatctcaa
     2761 ttagatgcaa cagatattca gtcagagact gctagcatgg tgagcaaggg cgaggaggat
     2821 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     2881 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     2941 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     3001 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     3061 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     3121 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     3181 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     3241 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     3301 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     3361 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     3421 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     3481 ggcggcatgg acgagctgta caagtaa
//
