LOCUS       sequence-115677-        6827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conjugative cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Fischer HM, Babst M, Kaspar T, Acuna G, Arigoni F, Hennecke H
  TITLE     One member of a gro-ESL-like chaperonin multigene family in 
            Bradyrhizobium japonicum is co-regulated with symbiotic nitrogen 
            fixation genes.
  JOURNAL   EMBO J. 1993 Jul;12(7):2901-12.
  PUBMED    8101485
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1993-07"; volume: "12(7)"; pages: "2901-12"
COMMENT     Sequence Label: pSUP202pol4
FEATURES             Location/Qualifiers
     source          1..6827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             57..1247
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     CDS             1888..2079
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2073..2095)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2181..2321
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2236..2255
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2336..2353)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2507..3095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2587..2606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3716..3732
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3716..3732
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(5044..5415)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            5448..5557
                     /note="incP origin of transfer"
     promoter        join(6808..6813,6814..6827,1..3,4..9)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga
       61 aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag
      121 gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg
      181 ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg
      241 ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg
      301 gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg
      361 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      421 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
      481 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
      541 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
      601 aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct
      661 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
      721 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
      781 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
      841 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
      901 ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
      961 tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
     1021 ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
     1081 ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct
     1141 cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc
     1201 tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg
     1261 gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac
     1321 tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag
     1381 ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct
     1441 cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc
     1501 accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac
     1561 aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc
     1621 ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc
     1681 tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg
     1741 catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc
     1801 agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag
     1861 aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc ccttaacatg
     1921 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg
     1981 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc
     2041 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     2101 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     2161 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     2221 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2281 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     2341 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2401 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2461 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2521 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2581 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2641 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2701 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2761 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2821 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2881 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2941 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3001 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3061 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3121 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3181 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3241 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3301 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3361 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3421 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3481 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3541 gttcgccagt taatagtttg cgcaacgttg ttgccattgc cgcggttcgg tgatgccacg
     3601 atcctcgccc tgctggcgaa gatcgaagag aagcaggacg agcttggcaa ggtcatgatg
     3661 ggcgtggtcc gcccgagggc agagccatga ctttttccac cgcggtggcg gccgctctag
     3721 aactagtgga tcccccgggc tgcaggaatt ccgtatggca atgaaagacg gtgagctggt
     3781 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     3841 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     3901 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     3961 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     4021 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     4081 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     4141 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta
     4201 aggcagttat tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg
     4261 gatgaatggc agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt
     4321 taaatagccg cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg
     4381 accgtgtgct tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata
     4441 attgacgata tgatccagcc gaccaggctt tccacgcccg cgtgccgctc catgtcgttc
     4501 gcgcggttct cggaaacgcg ctgccgcgtt tcgtgattgt cacgctcaag cccgtagtcc
     4561 cgttcgagcg tcgcgcagag gtcagcgagg gcgcggtagg cccgatacgg ctcatggatg
     4621 gtgtttcggg tcgggtgaat cttgttgatg gcgatatgga tgtgcaggtt gtcggtgtcg
     4681 tgatgcacgg cactgacgcg ctgatgctcg gcgaagccaa gcccagcgca gatgcggtcc
     4741 tcaatcgcgc gcaacgtctc cgcgtcgggc ttctctcccg cgcggaagct aaccagcagg
     4801 tgataggtct tgtcggcctc ggaacgggtg ttgccgtgct gggtcgccat cacctcggcc
     4861 atgacagcgg gcagggtgtt tgcctcgcag ttcgtgacgc gcacgtgacc caggcgctcg
     4921 gtcttgcctt gctcgtcggt gatgtacttc accagctccg cgaagtcgct cttcttgatg
     4981 gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta gcggctaaaa
     5041 aagtcatggc tctgccctcg ggcggaccac gcccatcatg accttgccaa gctcgtcctg
     5101 cttctcttcg atcttcgcca gcagggcgag gatcgtggca tcaccgaacc gcgccgtgcg
     5161 cgggtcgtcg gtgagccaga gtttcagcag gccgcccagg cggcccaggt cgccattgat
     5221 gcgggccagc tcgcggacgt gctcatagtc cacgacgccc gtgattttgt agccctggcc
     5281 gacggccagc aggtaggccg acaggctcat gccggccgcc gccgcctttt cctcaatcgc
     5341 tcttcgttcg tctggaaggc agtacacctt gataggtggg ctgcccttcc tggttggctt
     5401 ggtttcatca gccatccgct tgccctcatc tgttacgccg gcggtagccg gccagcctcg
     5461 cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca
     5521 cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca
     5581 aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata
     5641 taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaaa agcgctgctt
     5701 ccctgctgtt ttgtggaata tctaccgact ggaaacaggc aaatgcagga aattactgaa
     5761 ctgaggggac aggcgagaga cgatgccaaa gagctacacc gacgagctgg ccgagtgggt
     5821 tgaatcccgc gcggccaaga agcgccggcg tgatgaggct gcggttgcgt tcctggcggt
     5881 gagggcggat gtcgaggcgg cgttagcgtc cggctatgcg ctcgtcacca tttgggagca
     5941 catgcgggaa acggggaagg tcaagttctc ctacgagacg ttccgctcgc acgccaggcg
     6001 gcacatcaag gccaagcccg ccgatgtgcc cgcaccgcag gccaaggctg cggaacccgc
     6061 gccggcaccc aagacgccgg agccacggcg gccgaagcag gggggcaagg ctgaaaagcc
     6121 ggcccccgct gcggccccga ccggcttcac cttcaaccca acaccggaca aaaaggatct
     6181 actgtaatgg cgaaaattca catggttttg cagggcaagg gcggggtcgg caagtcggcc
     6241 atcgccgcga tcattgcgca gtacaagatg gacaaggggc agacaccctt gtgcatcgac
     6301 accgacccgg tgaacgcgac gttcgagggc tacaaggccc tgaacgtccg ccggctgaac
     6361 atcatggccg gcgacgaaat taactcgcgc aacttcgaca ccctggtcga gctgattgcg
     6421 ccgaccaagg atgacgtggt gatcctctac gccggacgca tcgtggccgg catcaccggc
     6481 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatccttga
     6541 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc
     6601 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg
     6661 aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc
     6721 tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt
     6781 ccgaataccg caagcgacag gccatgtttg acagcttatc atcgata
//
