LOCUS       sequence-115679-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracycline biosensor. High copy plasmid that expresses GFP 
            proportional to tetracycline concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-tetR
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(268..293,294..299,300..316,317..322,
                     323..341))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    298..316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    323..341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(503..589)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     742..759
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tggtccgggt acgagcgaag cactgaacac cgtaggtcag ggtggtaacc agggtcggcc
       61 acggaaccgg cagtttaccg gtggtgcaga tgaatttcag ggtcagttta ccgttggtag
      121 cgtcaccttc accttcacca cgaacagaga atttgtgacc gttaacgtca ccgtccagtt
      181 caaccaggat cggaacaaca ccggtgaaca gttcttcacc tttacgcata tgtatatctc
      241 cttcttaaag ttaaatttaa tgaattcggt cagtgcgtcc tgctgatgtg ctcagtatct
      301 ctatcactga tagggatgtc aatctctatc actgataggg actcgagtcg gccttacttg
      361 ctagcagagt ttgtagaaac gcaaaaaggc catcctgacg gatggccttc tgcttaattt
      421 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa
      481 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
      541 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag
      601 ttccctactc tcgcatgggg agaccccaca ctaccatcgg cgctacggcg tttcacttct
      661 gagttcggca tggggtcagg tgggaccacc gcgctactgc cgccaggcaa attctgtttt
      721 atcagaccgc ttctgcgttc tgatttaatc tgtatcaggc tgaaaatctt ctctcatccg
      781 ccaaaacagc ctgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaa
//
