LOCUS       sequence-115682-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLPo recombinase from the broadly active CAG
            promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBCV
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             15..35
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcgccaccat ggctcctaag aagaagagga aggtgatgag ccagttcgac atcctgtgca
       61 agaccccccc caaggtgctg gtgcggcagt tcgtggagag attcgagagg cccagcggcg
      121 agaagatcgc cagctgtgcc gccgagctga cctacctgtg ctggatgatc acccacaacg
      181 gcaccgccat caagagggcc accttcatga gctacaacac catcatcagc aacagcctga
      241 gcttcgacat cgtgaacaag agcctgcagt tcaagtacaa gacccagaag gccaccatcc
      301 tggaggccag cctgaagaag ctgatccccg cctgggagtt caccatcatc ccttacaacg
      361 gccagaagca ccagagcgac atcaccgaca tcgtgtccag cctgcagctg cagttcgaga
      421 gcagcgagga ggccgacaag ggcaacagcc acagcaagaa gatgctgaag gccctgctgt
      481 ccgagggcga gagcatctgg gagatcaccg agaagatcct gaacagcttc gagtacacca
      541 gcaggttcac caagaccaag accctgtacc agttcctgtt cctggccaca ttcatcaact
      601 gcggcaggtt cagcgacatc aagaacgtgg accccaagag cttcaagctg gtgcagaaca
      661 agtacctggg cgtgatcatt cagtgcctgg tgaccgagac caagacaagc gtgtccaggc
      721 acatctactt tttcagcgcc agaggcagga tcgaccc
//
