LOCUS       sequence-115685-       10122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPaint target selector TUBB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label:  pCAS9-mCherry-TUBB
FEATURES             Location/Qualifiers
     source          1..10122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             938..1354
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1284..1306
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1322..1338
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1365..1613
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1365..1385
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1536..1555
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1634..1709
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        1750..2129
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2130..2333
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2284..2304
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2394..2403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2403..2426
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2427..3131
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2569..2587)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2837..2856
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3156..3209
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3210..7313
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3210..7313
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7326..7346
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     LTR             7403..7996
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8197..8214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8368..8956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8448..8467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9134..9925,9926..9994))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9757..9776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9995..10099)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ctagttagct aactagctct gtatctggcg gacccgtggt
      661 ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag ggactttggg
      721 ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga
      781 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg
      841 ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct
      901 ctgtctgact gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt
      961 aagtttgacc ttagatcact ggaaagatgt cgagcggctc gctcacaacc agtcggtaga
     1021 tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg
     1081 atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt
     1141 ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt
     1201 ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct
     1261 tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc
     1321 cctttatcca gccctcactc cttctctagg cgccggccgg atccgagggc ctatttccca
     1381 tgattccttc atatttgcat atacgataca aggctgttag agagataatt ggaattaatt
     1441 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     1501 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     1561 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgggtgag
     1621 gaggccgaag agggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     1681 aacttgaaaa agtggcaccg agtcggtgct ttttttcgaa ttccgatgta cgggccagat
     1741 atacgcgttg acattgatta ttgtctagtt attaatagta atcaattacg gggtcattag
     1801 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     1861 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     1921 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     1981 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2041 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2101 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2161 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2221 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2281 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
     2341 taactagaga acccactgct tactggctta tcgaaattaa gcttccgcgt acggccacca
     2401 tggattataa ggatgacgac gataaggtga gcaagggcga ggaggataac atggccatca
     2461 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     2521 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     2581 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     2641 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     2701 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     2761 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     2821 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     2881 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     2941 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     3001 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     3061 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     3121 agctgtacaa ggtcgctacc atcgagggat ccggagaggg cagaggaagt ctgctaacat
     3181 gcggtgacgt cgaggagaat cctggcccaa tggacaagaa gtacagcatc ggcctggaca
     3241 tcggcaccaa cagcgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
     3301 agttcaaggt gctgggcaac accgaccgcc acagcatcaa gaagaacctg atcggcgccc
     3361 tgctgttcga cagcggcgag accgccgagg ccacccgcct gaagcgcacc gcccgccgcc
     3421 gctacacccg ccgcaagaac cgcatctgct acctgcagga gatcttcagc aacgagatgg
     3481 ccaaggtgga cgacagcttc ttccaccgcc tggaggagag cttcctggtg gaggaggaca
     3541 agaagcacga gcgccacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
     3601 agtaccccac catctaccac ctgcgcaaga agctggtgga cagcaccgac aaggccgacc
     3661 tgcgcctgat ctacctggcc ctggcccaca tgatcaagtt ccgcggccac ttcctgatcg
     3721 agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga
     3781 cctacaacca gctgttcgag gagaacccca tcaacgccag cggcgtggac gccaaggcca
     3841 tcctgagcgc ccgcctgagc aagagccgcc gcctggagaa cctgatcgcc cagctgcccg
     3901 gcgagaagaa gaacggcctg ttcggcaacc tgatcgccct gagcctgggc ctgaccccca
     3961 acttcaagag caacttcgac ctggccgagg acgccaagct gcagctgagc aaggacacct
     4021 acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgttcc
     4081 tggccgccaa gaacctgagc gacgccatcc tgctgagcga catcctgcgc gtgaacaccg
     4141 agatcaccaa ggcccccctg agcgccagca tgatcaagcg ctacgacgag caccaccagg
     4201 acctgaccct gctgaaggcc ctggtgcgcc agcagctgcc cgagaagtac aaggagatct
     4261 tcttcgacca gagcaagaac ggctacgccg gctacatcga cggcggcgcc agccaggagg
     4321 agttctacaa gttcatcaag cccatcctgg agaagatgga cggcaccgag gagctgctgg
     4381 tgaagctgaa ccgcgaggac ctgctgcgca agcagcgcac cttcgacaac ggcagcatcc
     4441 cccaccagat ccacctgggc gagctgcacg ccatcctgcg ccgccaggag gacttctacc
     4501 ccttcctgaa ggacaaccgc gagaagatcg agaagatcct gaccttccgc atcccctact
     4561 acgtcggccc cctggcccgc ggcaacagcc gcttcgcctg gatgacccgc aagagcgagg
     4621 agaccatcac cccctggaac ttcgaggagg tggtggacaa gggcgccagc gcccagagct
     4681 tcatcgagcg catgaccaac ttcgacaaga acctgcccaa cgagaaggtg ctgcccaagc
     4741 acagcctgct gtacgagtac ttcaccgtgt acaacgagct gaccaaggtg aagtacgtga
     4801 ccgagggcat gcgcaagccc gccttcctga gcggcgagca gaagaaggcc atcgtggacc
     4861 tgctgttcaa gaccaaccgc aaggtgaccg tgaagcagct gaaggaggac tacttcaaga
     4921 agatcgagtg cttcgacagc gtggagatca gcggcgtgga ggaccgcttc aacgccagcc
     4981 tgggcaccta ccacgacctg ctgaagatca tcaaggacaa ggacttcctg gacaacgagg
     5041 agaacgagga catcctggag gacatcgtgc tgaccctgac cctgttcgag gaccgcgaga
     5101 tgatcgagga gcgcctgaag acctacgccc acctgttcga cgacaaggtg atgaagcagc
     5161 tgaagcgccg ccgctacacc ggctggggcc gcctgagccg caagctgatc aacggcatcc
     5221 gcgacaagca gagcggcaag accatcctgg acttcctgaa gagcgacggc ttcgccaacc
     5281 gcaacttcat gcagctgatc cacgacgaca gcctgacctt caaggaggac atccagaagg
     5341 cccaggtgag cggccagggc gacagcctgc acgagcacat cgccaacctg gccggcagcc
     5401 ccgccatcaa gaagggcatc ctgcagaccg tgaaggtggt ggacgagctg gtgaaggtga
     5461 tgggccgcca caagcccgag aacatcgtga tcgagatggc ccgcgagaac cagaccaccc
     5521 agaagggcca gaagaacagc cgcgagcgca tgaagcgcat cgaggagggc atcaaggagc
     5581 tgggcagcca gatcctgaag gagcaccccg tggagaacac ccagctgcag aacgagaagc
     5641 tgtacctgta ctacctgcag aacggccgcg acatgtacgt ggaccaggag ctggacatca
     5701 accgcctgag cgactacgac gtggaccaca tcgtgcccca gagcttcctg aaggacgaca
     5761 gcatcgacaa caaggtgctg acccgcagcg acaagaaccg cggcaagagc gacaacgtgc
     5821 ccagcgagga ggtggtgaag aagatgaaga actactggcg ccagctgctg aacgccaagc
     5881 tgatcaccca gcgcaagttc gacaacctga ccaaggccga gcgcggcggc ctgagcgagc
     5941 tggacaaggc cggcttcatc aagcgccagc tggtggagac ccgccagatc accaagcacg
     6001 tggcccagat cctggacagc cgcatgaaca ccaagtacga cgagaacgac aagctgatcc
     6061 gcgaggtgaa ggtgatcacc ctgaagagca agctggtgag cgacttccgc aaggacttcc
     6121 agttctacaa ggtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg
     6181 ccgtggtggg caccgccctg atcaagaagt accccaagct ggagagcgag ttcgtgtacg
     6241 gcgactacaa ggtgtacgac gtgcgcaaga tgatcgccaa gagcgagcag gagatcggca
     6301 aggccaccgc caagtacttc ttctacagca acatcatgaa cttcttcaag accgagatca
     6361 ccctggccaa cggcgagatc cgcaagcgcc ccctgatcga gaccaacggc gagaccggcg
     6421 agatcgtgtg ggacaagggc cgcgacttcg ccaccgtgcg caaggtgctg agcatgcccc
     6481 aggtgaacat cgtgaagaag accgaggtgc agaccggcgg cttcagcaag gagagcatcc
     6541 tgcccaagcg caacagcgac aagctgatcg cccgcaagaa ggactgggac cccaagaagt
     6601 acggcggctt cgacagcccc accgtggcct acagcgtgct ggtggtggcc aaggtggaga
     6661 agggcaagag caagaagctg aagagcgtga aggagctgct gggcatcacc atcatggagc
     6721 gcagcagctt cgagaagaac cccatcgact tcctggaggc caagggctac aaggaggtga
     6781 agaaggacct gatcatcaag ctgcccaagt acagcctgtt cgagctggag aacggccgca
     6841 agcgcatgct ggccagcgcc ggcgagctgc agaagggcaa cgagctggcc ctgcccagca
     6901 agtacgtgaa cttcctgtac ctggccagcc actacgagaa gctgaagggc agccccgagg
     6961 acaacgagca gaagcagctg ttcgtggagc agcacaagca ctacctggac gagatcatcg
     7021 agcagatcag cgagttcagc aagcgcgtga tcctggccga cgccaacctg gacaaggtgc
     7081 tgagcgccta caacaagcac cgcgacaagc ccatccgcga gcaggccgag aacatcatcc
     7141 acctgttcac cctgaccaac ctgggcgccc ccgccgcctt caagtacttc gacaccacca
     7201 tcgaccgcaa gcgctacacc agcaccaagg aggtgctgga cgccaccctg atccaccaga
     7261 gcatcaccgg cctgtacgag acccgcatcg acctgagcca gctgggcggc gacagccgcg
     7321 ccgaccccaa gaagaagcgc aaggtgtgag cggccgcatc gataaaataa aagattttat
     7381 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     7441 aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat
     7501 caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     7561 gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga
     7621 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     7681 ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     7741 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     7801 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc
     7861 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     7921 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     7981 gcgggggtct ttcatttccg acttgtggtc tcgctgcctt gggagggtct cctctgagtg
     8041 attgactacc cgtcagcggg ggtcttcaca tgcagcatgt atcaaaatta atttggtttt
     8101 ttttcttaag tatttacatt aaatggccat agttgcatta atgaatcggc caacgcgcgg
     8161 ggagaggcgg tttgcgtatt ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8221 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8281 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8341 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8401 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8461 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8521 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8581 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8641 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8701 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8761 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     8821 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8881 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8941 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9001 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9061 ccttttaaat taaaaatgaa gtttgcggcc gcaaatcaat ctaaagtata tatgagtaaa
     9121 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9181 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9241 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9301 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9361 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9421 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9481 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9541 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9601 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9661 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9721 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     9781 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9841 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9901 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9961 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10021 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10081 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
