LOCUS       sequence-115686-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracycline biosensor. High copy plasmid that expresses GFP 
            proportional to tetracycline concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-tetR
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             38..46
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tatcacttta cttttatcta aacgagacat ttgtattacc tccttatttc aaagttaaac
       61 aaaattattt gtagagggaa accgttgtgg tctccctgaa tatagcctac gagccttatg
      121 catgcccgta aagttatcca gcaaccactc atagacctag ggcagcagat agggacgacg
      181 tggtgttagc tgtggtaaac gccatgggca ttaaaaaatg ccctcttggg ttatcaagag
      241 ggtcattata tttgctgagg agtgccgtta attaaggcgc aacgcaatta atgtaagtta
      301 gctcactcat tagggttatg ctagaactgg catgcatctt tgtacagttc gtccataccg
      361 tgggtgatac cagcagcggt aacgaattcc agcagaacca tgtggtcacg tttttcgttc
      421 gggtctttag acagaacaga ctgggtagac aggtagtggt tgtccggcaa cagaaccgga
      481 ccgtcaccga tcggggtgtt ctgctggtag tggtcagcca gctgaacaga accgtcttca
      541 acgttgtgac ggattttgaa gttagctttg ataccgtttt tctgtttgtc agcggtgatg
      601 taaacgttgt gagagttgaa gttgtattcc agtttgtgac ccaggatgtt accgtcttct
      661 ttgaagtcga tacctttcag ttcgatacgg ttaaccaggg tgtcaccttc gaatttaact
      721 tcagcacggg ttttgtaggt accgtcgtct ttgaaagaga tggtacgttc ctgaacgtaa
      781 ccttccggca tagcagattt gaagaagtcg tgctgtttca tgtggtccgg gtaacgagcg
      841 aagcactgaa caccgtaggt cagggtggta acca
//
