LOCUS       sequence-115687-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Acrylate biosensor. High copy plasmid that expresses GFP 
            proportional to acrylate concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-acuR
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(338..365)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(457..543)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     696..713
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 acgagcgaag cactgaacac cgtaggtcag ggtggtaacc agggtcggcc acggaaccgg
       61 cagtttaccg gtggtgcaga tgaatttcag ggtcagttta ccgttggtag cgtcaccttc
      121 accttcacca cgaacagaga atttgtgacc gttaacgtca ccgtccagtt caaccaggat
      181 cggaacaaca ccggtgaaca gttcttcacc tttacgcata tgtatatctc cttcttaaag
      241 ttaaatgttc cagaaataat agacggtatg gtctattaaa tcaaagtgcg gttgtgaagc
      301 gtcggcctta cttgctagca gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
      361 cttctgctta atttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
      421 ccgttgcttc gcaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
      481 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      541 ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac
      601 ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag
      661 gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa
      721 tcttctctca tccgccaaaa cagcctgagc aaaaggccag caaaaggcca ggaaccgtaa
      781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      841 tcgacgctca agtcagaggt ggcgaaaccc gacag
//
